Herein, we will discuss the research knowledge regarding SC-based therapy for the treatment of GB, focalising our attention on SCs and SC-released extracellular vesicles modified to express/load different antitumour payloads, as well as on SCs exploited as a diagnostic tool. Advantages and unresolved issues of anticancer SC-based therapy will also be considered. We sought to assess the correlation of corneal endothelial cell (CEC) density to alterations of collagen composition of Descemet membrane (DM) in advanced Fuchs endothelial corneal dystrophy (FECD) and to image such changes by slit-lamp biomicroscopy invivo. Prospective, observational consecutive case series. Fifty eyes (50 subjects) with advanced FECD were enrolled. After slit-lamp biomicroscopy and corneal Scheimpflug imaging, the Descemet endothelium complex (DEC) was retrieved during DM endothelial keratoplasty (DMEK) surgery. The expression of collagens I, III, and IV (COL I, COL III, and COL IV) and corresponding CEC density were analyzed by immunofluorescence flat mount-staining. Presence, diameter and surface area of collagen expression, and CEC density served as the main outcome measures. Immunofluorescence staining revealed central coherent collagen positive areas (mean surface area= 10mm ± 6mm ) corresponding to a fibrillar layer burying the guttae of DM in 84% (42/50) of DECs. CEC density overlying the fibrillar layer compared with the periphery was significantly reduced (-54.8%, P< .0001) with a steep decline of CEC density at its borders. Subgroup analysis revealed that the fibrillar layer may be imaged by slit-lamp biomicroscopy invivo with significant positive correlation of mean maximum diameter detected by slit-lamp biomicroscopy (d = 4.1mm ± 0.9mm) and by immunofluorescence staining (d = 4.7mm ± 1.1mm; r= 0.76; P= .001). A fibrillar layer with a clear geographic pattern marks areas of pronounced loss of CEC density in advanced FECD eyes and may be imaged by slit-lamp biomicroscopy invivo.A fibrillar layer with a clear geographic pattern marks areas of pronounced loss of CEC density in advanced FECD eyes and may be imaged by slit-lamp biomicroscopy in vivo. To compare lamina cribrosa curvature (LCC) in pseudoexfoliation glaucoma (PXG) and primary open-angle glaucoma (POAG) and to elucidate the factors associated with LCC in both groups. Retrospective, cross-sectional study. Forty eyes with PXG and visual field mean deviation-matched 40 eyes with POAG were included. Spectral domain optical coherence tomography enhanced depth imaging was used to acquire LC images. The LCC index (LCCI) was calculated as LCC depth/LCC width× 100. Calculated LCCI values were compared between the PXG and POAG groups. Logistic regression analysis was performed to investigate the factors related to the presence of PXG. Y-27632 ROCK inhibitor Factors associated with LCCI were determined by uni- and multivariable linear regression analyses in both groups. Visual field mean deviation (-11.7 ± 9.7 dB vs-1.0 ± 8.5 dB, P= .731) and retinal nerve fiber thickness (647.4 ± 22.7μm vs 68.3 ± 19.9μm, P= .857) did not differ between the 2 groups. However, the mean (8.8 ± 2.9 in PXG, 6.6 ± 1.9 in POAG) and sectoral LCCIs assessed at 0, 30, 60, 90, and 120° were significantly greater in the PXG than in the POAG group (all, P < .05), but not at 150° (P= .113). Logistic regression analysis revealed that higher average LCCI (odds ratio [OR]= 1.509 [95% confidence interval 1.180-2.047], P= .003) and older age (OR= 1.074 [95% confidence interval 1.017-1.147], P= .019) were associated with the presence of PXG. IOP was associated with mean LCCI only in the POAG group (β= 0.150 [95% confidence interval 0.018-0.281], P= .027) while none of the factors showed association with LCCI in PXG. PXG eyes had more steeply curved LC than POAG eyes with a similar level of glaucoma severity. The clinical implication(s) of steeper LCC in PXG should be investigated in a forthcoming study.PXG eyes had more steeply curved LC than POAG eyes with a similar level of glaucoma severity. The clinical implication(s) of steeper LCC in PXG should be investigated in a forthcoming study. To present morphogeometric and volumetric characteristics of the cornea and its diagnostic value in pediatric patients with keratoconus (KC) using 3-dimensional (3-D) corneal modeling. Cross-sectional study. This single-center (VISSUM Innovation, Alicante, Spain) study comprised 49 eyes of 49 pediatric patients (age ≤16 years) with KC and 31 eyes of 31 healthy pediatric controls. Eyes were graded as early (n= 21) and mild KC (n= 28) based on the RETICS (Thematic Network for Co-Operative Research in Health) classification system. The 3-D corneal model was generated using raw topographic data. Deviation of anterior (D ) and posterior (D ) apex and minimum thickness points (D , D ), D -D difference, total corneal volume (V ), volumetric distribution (VOL , VOL , and VOL ), and percentage of relative volume increase (VOL , VOL , and VOL ) between 2 consecutive radii centered to anterior/posterior apex and thinnest point were evaluated. D and D -D difference were higher in the early and mild KC groups compared to the control group (P < .05). Eyes with early and mild KC had decreased V compared with the control group (P < .05). D , D -D difference, and VOL between 1.0 and 1.4mm diameters had area under receiver operating characteristics curve (AUROC) values over 0.93 in discrimination of early KC from normal. This is the first study presenting morphogeometric and volumetric characterization of cornea in pediatric patients with early and mild KC using a 3-D corneal model. Integration of the morphogeometric and volumetric parameters to topography software can add value in early detection of KC in pediatric patients.This is the first study presenting morphogeometric and volumetric characterization of cornea in pediatric patients with early and mild KC using a 3-D corneal model. Integration of the morphogeometric and volumetric parameters to topography software can add value in early detection of KC in pediatric patients.