Bacterial cell division is driven by the polymerization of the GTPase FtsZ into a contractile structure, the so-called Z-ring. This essential process involves proteins that modulate FtsZ dynamics and hence the overall Z-ring architecture. Actinobacteria like Streptomyces and Mycobacterium lack known key FtsZ-regulators. Here we report the identification of SepH, a conserved actinobacterial protein that directly regulates FtsZ dynamics. We show that SepH is crucially involved in cell division in Streptomyces venezuelae and that it binds FtsZ via a conserved helix-turn-helix motif, stimulating the assembly of FtsZ protofilaments. Comparative in vitro studies using the SepH homolog from Mycobacterium smegmatis further reveal that SepH can also bundle FtsZ protofilaments, indicating an additional Z-ring stabilizing function in vivo. We propose that SepH plays a crucial role at the onset of cytokinesis in actinobacteria by promoting the assembly of FtsZ filaments into division-competent Z-rings that can go on to mediate septum synthesis. The maintenance of wakefulness test (MWT) is used to objectively evaluate an individual's ability to remain awake, however microsleeps are not included in the assessment. We aimed to determine if microsleep data prior to sleep onset assisted in interpretation of ability to maintain wakefulness across a range of typical patient groups. Forty-eight patients referred for overnight polysomnography (PSG) and subsequent MWT were included. Patients were divided into three groups (treated OSA, untreated OSA or treated Idiopathic Hypersomnia or Narcolepsy (treated IH/Narc)) based on prior medical diagnosis. Demographics, clinical characteristics, PSG and MWT variables including frequency, distribution, duration and latency of microsleeps were compared between groups. Microsleeps were observed in MWT trials significantly more frequently in treated IH/Narc patients over the course of the day (0.34 ± 0.06 vs 0.07 ± 0.02 microsleeps/min; p < 0.001) and in untreated OSA patients toward the end of the day (0.31 ± 0.06 vs 0.05 ± 0.02 microsleeps/min; p<0.001) compared to the treated OSA group. Microsleeps were often observed in series and earlier in treated IH/Narc (10.9 ± 1.6 minutes) and untreated OSA patients (16.2 ± 2.7 minutes) compared to the treated OSA group (24.9 ± 3.0 minutes; p<0.05), and if taken into consideration would increase the proportion of patients demonstrating inability to maintain wakefulness by 33% and 22% respectively. MWT performance varies significantly across patient groups. Microsleep analysis prior to sleep onset may be a more sensitive measure of patient daytime wakefulness than sleep latency alone, and should be considered in MWT assessment.MWT performance varies significantly across patient groups. Microsleep analysis prior to sleep onset may be a more sensitive measure of patient daytime wakefulness than sleep latency alone, and should be considered in MWT assessment. To explore the association of continuous positive airway pressure (CPAP) compliance with clinical outcomes in patients with type 2 diabetes (T2D) and obstructive sleep apnea (OSA) in a real-world setting. This was a retrospective study of patients with T2D diagnosed with OSA between 2010 and 2017. CPAP compliance (usage for ≥4 h/night for ≥70% of nights) was determined from the first CPAP report following the polysomnography. Data including estimated glomerular filtration rate (eGFR), hemoglobin A1C (HbA1c), systolic and diastolic blood pressure (SBP, DBP), lipid panel, and incident cardiovascular/peripheral vascular/cerebrovascular events (CVD, PVD, CVA) were extracted from medical records. IOX1 datasheet Mixed-effects linear regression modeling of longitudinal repeated measures within patients was utilized for continuous outcomes, and logistic regression modeling was used for binary outcomes. Models were controlled for age, sex, body mass index, medications, and baseline levels of outcomes. Of the 1,295 patients, 260 (20.7%) were CPAP compliant, 318 (24.5%) were CPAP non-compliant, while 717 (55.3%) had insufficient data. The follow up period was on average 2.5 (1.7) years. Compared to those who were CPAP non-compliant, those who were compliant had a significantly lower SBP (β=-1.95 mmHg, p=0.001) and DBP (β=-2.33 mmHg, p<0.0001). Among the CPAP-compliant patients, a 17% greater CPAP compliance was associated with a 2 mmHg lower SBP. Lipids, HbA1c, eGFR and incident CVD/PVD/CVA were not different between the two groups. Achieving CPAP compliance in T2D patients with OSA was associated with significantly lower blood pressure. Greater CPAP use within compliant patients was associated with lower SBP.Achieving CPAP compliance in T2D patients with OSA was associated with significantly lower blood pressure. Greater CPAP use within compliant patients was associated with lower SBP. Evaluate changes in mood, perceived need for help, and task enjoyment after participation in a one-time Oncology on Canvas expressive arts workshop intervention. Mixed methods design. Sample military-veteran oncology survivors and their adult `ohana (family members, caregivers, friends, healthcare providers, volunteers). Measurement tools Emotion Thermometers, Multiple Affect Adjective Checklist-Revised, Task Enjoyment Question, semi-structured interviews, and self-reflective narratives. Colaizzi's Phenomenological Method was used to conduct a thematic analysis. Significant improvements in mood, increased task enjoyment, and decreased perceived need for help, with continued effects for up to 30-60 days post-intervention. A one-time expressive arts workshop intervention can provide a window for oncology survivors and their adult `ohana to reflect on a stressful experience. Psychosocial healthcare providers (APRNs, nurse educators, LCSWs, psychologists) who are not professional art therapists can effectively implement expressive arts workshops as a cost-effective intervention for oncology survivors and their `ohana.Psychosocial healthcare providers (APRNs, nurse educators, LCSWs, psychologists) who are not professional art therapists can effectively implement expressive arts workshops as a cost-effective intervention for oncology survivors and their `ohana.