Yellow pea and faba bean are potential candidates to replace soybean-based ingredients due to their suitability for cultivation in the northern hemisphere, non-genetically modified organisms cultivation practice and low risk of allergenicity. This study examined the functionality of local yellow pea and faba bean protein isolates/concentrate as meat analogue products. The most critical factors affecting the texture properties of meat analogue were also determined. Extrusion was used to produce high-moisture meat analogues (HMMAs) from yellow pea and faba bean protein isolates/concentrates and HMMAs with fibrous layered structures was successfully produced from both imported commercial and local sources. The texture properties of the HMMA produced were mainly affected by the ash, fiber and protein content and water-holding capacity of the source protein. Three extrusion process parameters (target moisture content, extrusion temperature, screw speed), also significantly affected HMMA texture. In conclusion, functional HMMA can be produced using protein isolates derived from locally grown pulses.Real-time estimation of temperatures in indoor environments is critical for several reasons, including the upkeep of comfort levels, the fulfillment of legal requirements, and energy efficiency. Unfortunately, setting an adequate number of sensors at the desired locations to ensure a uniform monitoring of the temperature in a given premise may be troublesome. Virtual sensing is a set of techniques to replace a subset of physical sensors by virtual ones, allowing the monitoring of unreachable locations, reducing the sensors deployment costs, and providing a fallback solution for sensor failures. In this paper, we deal with temperature monitoring in an open space office, where a set of physical sensors is deployed at uneven locations. Our main goal is to develop a black-box virtual sensing framework, completely independent of the physical characteristics of the considered scenario, that, in principle, can be adapted to any indoor environment. We first perform a systematic analysis of various distance metrics that can be used to determine the best sensors on which to base temperature monitoring. Then, following a genetic programming approach, we design a novel metric that combines and summarizes information brought by the considered distance metrics, outperforming their effectiveness. Thereafter, we propose a general and automatic approach to the problem of determining the best subset of sensors that are worth keeping in a given room. Leveraging the selected sensors, we then conduct a comprehensive assessment of different strategies for the prediction of temperatures observed by physical sensors based on other sensors' data, also evaluating the reliability of the generated outputs. The results show that, at least in the given scenario, the proposed black-box approach is capable of automatically selecting a subset of sensors and of deriving a virtual sensing model for an accurate and efficient monitoring of the environment.Past research has found that a perceived meaning in life can act as a protective factor against adverse mental health symptomology, while also providing coping resources to buffer against the impact of negative life events. The current research investigated how the impact of self-perceived meaning in life as well as its predictors interact with stressors and worry related to the COVID-19 pandemic. We collected survey based data (n = 260) from Australian participants during the pandemic, measuring their meaning in life, orientation to differing life goals and COVID-19 related stressors via the impact of events scale. We found that meaning in life predicted less stress and worry associated with COVID-19. We also found that intrinsic based aspirations related positively to meaning in life within this context whereas extrinsic based goals related negatively to it, although these aspirations were not significant in reducing the stressors associated with COVID-19. These results reinforce past findings that meaning in life can effectively buffer against the impact of negative life events such as the COVID-19 pandemic. They also suggest that intrinsic based aspirations centred on relationships and self-acceptance may be an important mechanism in how people choose to pursue life meaning during uncertain life events.Resistance is threatening the effectiveness of insecticide-based interventions in use for malaria control. Pinpointing genes associated with resistance is crucial for evidence-based resistance management targeting the major malaria vectors. see more Here, a combination of RNA-seq based genome-wide transcriptional analysis and RNA-silencing in vivo functional validation were used to identify key insecticide resistance genes associated with DDT and DDT/permethrin cross-resistance across Africa. A cluster of glutathione-S-transferase from epsilon group were found to be overexpressed in resistant populations of Anopheles funestus across Africa including GSTe1 [Cameroon (fold change, FC 2.54), Ghana (4.20), Malawi (2.51)], GSTe2 [Cameroon (4.47), Ghana (7.52), Malawi (2.13)], GSTe3 [Cameroon (2.49), Uganda (2.60)], GSTe4 in Ghana (3.47), GSTe5 [Ghana (2.94), Malawi (2.26)], GSTe6 [Cameroun (3.0), Ghana (3.11), Malawi (3.07), Uganda (3.78)] and GSTe7 (2.39) in Ghana. Validation of GSTe genes expression profiles by qPCR confirmed that the genes are differentially expressed across Africa with a greater overexpression in DDT-resistant mosquitoes. RNAi-based knock-down analyses supported that five GSTe genes are playing a major role in resistance to pyrethroids (permethrin and deltamethrin) and DDT in An. funestus, with a significant recovery of susceptibility observed when GSTe2, 3, 4, 5 and GSTe6 were silenced. These findings established that GSTe3, 4, 5 and 6 contribute to DDT resistance and should be further characterized to identify their specific genetic variants, to help design DNA-based diagnostic assays, as previously done for the 119F-GSTe2 mutation. This study highlights the role of GSTes in the development of resistance to insecticides in malaria vectors and calls for actions to mitigate this resistance.