87-0.99. CONCLUSION Methylation analysis performs excellent in all urine fractions for cervical cancer detection. Our results indicate the potential of CIN3 detection by urinary methylation analysis, and demonstrate that urine sediment performs best to detect CIN3. The potential energy surfaces of the H2S binding to iron-porphyrin (FeP) with the imidazole (Im) ligand via intersystem crossings are investigated by using density functional theory. The minimum energy intersystem crossing point (MEISCP) between the quintet and triplet states (MEISCPTQ) for the Fe(II)P(Im)-H2S complex is located at a Fe-S distance of 3.39 Å with only 1.1 kcal/mol above the quintet state minimum. The second spin-crossover point, where a change from the triplet to the singlet state occurs, comes at a much shorter Fe-S distance of 2.79 Å, and the MEISCPST is located at 3.7 kcal/mol above the triplet state minimum. The nature of the chemical bonding along the Fe-S reaction coordinate from the ground state singlet to the quintet state along the path to the separated species is analyzed. this website An inspection of the vibrational modes reveals that the largest contribution to the triplet-quintet transition around the quintet and triplet state minimum comes from the symmetric shrinking of the pyrrole units of the porphyrin ring, indicating that the related reaction coordinate plays a main role in the intersystem crossing. The fully optimized structures of the Fe(II)P(Im)-HS- complex corresponding to three different spin multiplicities (M = 1, 3, 5) are characterized by a bent Fe-H-S conformation. The binding of the hydrosulfide anion to Fe(II)P(Im) in the quintet state induces a 0.2 Å displacement of the Fe atom out of the nitrogen porphyrin (Npyr) plane. The fully optimized structure of the ground state of Fe(II)P(Im)-HS- agrees well with experimental data for the corresponding heme models. The intricate and multifactorial nature of Alzheimer's disease (AD) requires the development of compounds able to hit different pathophysiological targets, such as cholinergic dysfunction, deposits of amyloid beta (Aβ) peptide and metal dyshomeostasis. In order to continue the search for new anti-AD drugs, a design strategy was once more followed based on repositioning donepezil (DNP) drug, by ortho-attaching a benzylpiperidine mimetic of DNP moiety to a hydroxyphenyl-benzimidazole (BIM) chelating unit (compound 1). Herein, compound 1 and a positional isomer 2 are compared in terms of their potential multiple properties both present good acetylcholinesterase (AChE) inhibition (low μmolar range) and are moderate/good inhibitors of Aβ self- and Cu-mediated aggregation, the inhibition process being mainly due to ligand intercalation between the β-sheets of the fibrils; compound 1 has a higher chelating capacity towards Cu2+ and Zn2+ (pCu = 14.3, pZn = 6.4, pH 7.4, CL/CM = 10, CM = 10-6 M) than 2 (pCu = 10.7, pZn = 6.3), attributed to its ability to establish a tridentate (N,O,O) coordination to the metal ion. Both compounds are eligible as drug candidates for oral administration but compound 1 shows improved neuroprotective role by completely preventing Aβ-induced cell toxicity. BACKGROUND The operative microscope, a commonly used tool in neurosurgery, is critical in many supratentorial tumor cases. However, use of operating microscope for supratentorial tumor varies by surgeon. OBJECTIVES To assess complication rates, readmissions, and costs associated with operative microscope use in supratentorial resections. METHODS A retrospective analysis was conducted using a national administrative database to identify patients with glioma or brain metastases who underwent supratentorial resection between 2007 and 2016. Univariate and multivariate analyses were used to assess 30-day complications, readmissions and costs between patients who underwent resection with and without use of microscope. RESULTS The cohort included 12058 glioma patients and 5433 metastasis patients. Rates of microscope use varied by state from 19.0% to 68.6%. Microscope use was associated with $5228.9 in additional costs of index hospitalization among glioma patients (p less then 0.001), and $2824.0 among metastasis patients (p less then 0.001). Rates of intraoperative cerebral edema were lower among the microscope cohort than among the non-microscope cohort (p less then 0.027). Microscope use was associated with a slight reduction in 30-day rates of neurological complications (14.7% vs. 16.7%, p = 0.048), specifically in nonspecific cerebrovascular complications. There were no differences in rates of other complications, readmissions, or 30-day postoperative costs. CONCLUSIONS Use of operative microscope for supratentorial resections varies by state and is associated with higher cost of surgery. Microscope use may be associated with lower rates of intraoperative cerebral edema and some cerebrovascular complications, but is not associated with significant differences in other complications, readmissions, or 30-day costs. BACKGROUND Bibliometric indexes are often used to evaluate and compare neurosurgeons and departments, and they were shown to correlate with academic rank and department reputation. We evaluated academic productivity of neurosurgeons practicing in departments accredited by the Joint Residency Advisory and Accreditation Committee (JRAAC) and European Association of Neurological Surgeons (EANS). METHODS In this cross-sectional study we calculated number of publications, citations, h-index and m-quotient from the Pubmed/MEDLINE and Web of Knowledge databases for 285 neurosurgeons affiliated with 19 department that are accredited or in-progress of accreditation by the JRAAAC. Academic productivity was compared as a function of academic rank, research degree. RESULTS Median number of publication in Pubmed/MEDLINE and Web of Knowledge indexed journals were 13 (range 0-352) and 15 (range 0-323), respectively. Median h-index was 4 (range 0-41) and median m-quotient was 0.56 (range 0-2.86). There was a significant variability between the studied departments in median number of publications and h-index (p=0.001). Professors and associate professors had significantly higher bibliometric indexes than neurosurgeon without academic rank (all p-values less then 0.001). Department chairmen had higher bibliometric indexes than other faculty members (all p-values less then 0.001). Neurosurgeons holding a research degree authored more publications and higher bibliometric indexes than faculty members not holding a research degree (p less then 0.001). CONCLUSIONS This is the first study evaluating academic productivity of neurosurgeons practicing in Europe and Turkey. Higher academic rank and advanced research degree were associated with greater academic productivity. Further studies exploring regional differences in academic productivity of European neurosurgeons are encouraged.