By combining Beauveria bassiana, an entomopathogenic fungus, with dsRNA-expressing bacteria carrying immune genes, we assessed the potential synergistic toxicity on the leaf beetle, Plagiodera versicolora (Coleoptera Chrysomelidae). The susceptibility of *P. versicolora* to the *B. bassiana* 476 strain, labeled Bb476, was our initial focus. Bb476-challenged beetle transcriptomes served as the foundation for identifying immune genes. Immune genes PGRP1, Toll1, DomelessSPN1, and Lysozyme were targeted subsequently by feeding dsRNA-expressing bacteria. The resulting gene silencing percentages were 714%, 390%, 720%, 490%, and 687%, respectively, for each gene. vx-770activator We also found that the mortality of P. versicolora was substantially higher when combined with Bb476 and immune-suppressive double-stranded RNA. This study, in its entirety, demonstrates the significance of insect immunity in defending against entomopathogens and consequently facilitates the creation of a more efficient integrated pest management strategy, one that effectively integrates both entomopathogens and immune-suppressing dsRNAs.Pest control frequently employs chlorantraniliprole (CAP), yet its environmental residues negatively impact the disease resistance of non-target silkworms. Detailed studies have unveiled a link between alterations in the insect gut's microbial population and the vulnerability of insects to pathogenic agents. This investigation analyzed how CAP exposure altered the silkworms' immune system's function and gut microbial ecosystem structure. The 96-hour exposure to low-concentration CAP caused a breakdown of the silkworm larva's peritrophic matrix (PM), permitting pathogenic bacteria to infiltrate the hemolymph. The activity of trehalase within the midgut was significantly reduced, while the activities of chitinase, -N-acetylglucosaminidase, and chitin deacetylase underwent a substantial increase, ultimately resulting in a diminished chitin concentration in the PM. The exposure to CAP further suppressed the expression of key genes involved in the Toll, IMD, and JAK/STAT pathways, consequently diminishing the expression of antimicrobial peptide (AMP) genes and impacting the composition of the gut microbial community. Consequently, following an infection by the conditional pathogen Enterobacter cloacae (E. Exposed individuals exhibiting CAP had a significantly lower body weight and a markedly higher mortality rate. Silkworms exposed to low levels of CAP experienced a decline in their biological defense systems, alterations in their gut microbial communities, and a heightened vulnerability to bacterial illnesses. The integrated results of these findings yielded a unique perspective regarding the safety implications of low-concentration CAP exposure in sericulture.A popular biopesticide, Bacillus thuringiensis (Bt), is environmentally sound and widely utilized. However, mirroring the limitations of other microbial pesticides, Bt's application is vulnerable to the detrimental effects of ultraviolet (UV) radiation, thereby significantly reducing its toxicity and persistence. In this study, metabolomic profiling was performed on both Bt LLP29 and its UV-resistant mutant, LLP29-M19, to investigate the mechanism of Bt's resistance to UV radiation. The analysis identified 61 metabolites that showed different levels of abundance. Twelve metabolic pathways, including the TCA cycle, experienced enrichment, exceeding the standard of a P-value below 0.05. RT-qPCR analysis of the TCA cycle key genes (icd1, citZ, citB, sdhA, sdhB, sdhC, fumA, and mdh) in Bt LL29-M19 revealed substantial downregulation, with percentage decreases of 8558%, 3702%, 7087%, 8597%, 7633%, 8315%, 8728%, and 3577%, as compared to their expression levels in Bt LLP29. Consistent with the trend of down-regulation, the activity of TCA cycle key enzymes was observed in Bt LLP29-M19. The enzyme activities of ICDH, SDH, and PDH in the LLP29-M19 strain displayed a significant reduction of 8628%, 4393%, and 8303%, respectively, when compared to those observed in Bt LLP29. The requirement of a reduced TCA cycle for Bt UV radiation resistance was established, supported by the addition of furfural and malonic acid inhibitors, respectively. RT-qPCR data on gene transcription levels revealed downregulation of key reactive oxygen species (ROS) generation pathways, such as EMP, and a simultaneous upregulation of major degrading enzyme activity. This suggests the TCA cycle strategically reduces ROS production to bolster Bt's anti-ultraviolet resistance. All these observations signify a strong correlation between metabolomics reprogramming and bolstering Bt's resistance to UV radiation.Widely distributed and causing significant economic losses, the striped flea beetle (SFB), Phyllotreta striolata (Fabricius), relentlessly attacks Brassicaceae plants globally. RNA interference (RNAi) stands as a promising technique for eco-friendly and sustainable solutions to the pest control challenge. This study examined the expression of nine housekeeping genes, including PsVATPA, PsHSP90, PsEF1A, PsRPL6, PsRPS24, PsActin, PsTUBA, PsRPS18, and PsRPL4, under four distinct conditions: organization, population, sex, and RNAi. PsEF1A and PsVATPA were determined to be the most suitable reference genes for RNA interference (RNAi) biological testing. In addition, twenty-four target genes were chosen to examine their RNA interference effects on SFB adult insects using double-stranded RNAs (dsRNAs). Remarkably, five of these genes—Ps-COPI, Ps-COPI, PsRPS18, Ps-COPI, and PsArf1COPI—demonstrated substantial mortality rates, ranging from 2800% to 7000%. Following treatment with target dsRNAs, either through feeding or injection, we observed a substantial reduction in gene transcript levels of the two most lethal genes, Ps-COPI and PsArf1COPI. This research indicated that dsRNAs, delivered through oral intake or by injection, prompted RNAi-mediated suppression of target gene expression, culminating in insect mortality. From the identified target genes, RNAi-based insecticides for SFB management warrant further exploration.The global agricultural industry faces a serious challenge in the form of gray mold, a widespread disease caused by Botrytis cinerea. Since the 1990s, anilinopyrimidine (AP) fungicides have been frequently used to combat the problem of gray mold. However, the rise of resistance in the *B. cinerea* fungus created a new obstacle to successful disease control. The ambiguous nature of AP resistance stems from the currently unknown mode of action. Our prior study identified a relationship between the E407K mutation in the Bcmdl1 gene and resistance to the antibiotic AP. In light of this mutation being the principal mechanism of AP resistance in our samples, it is vital to examine the fitness of E407K strains before developing anti-resistance management strategies. Field-resistant isolates harboring the E407K mutation, alongside strains carrying the E407K substitution generated through site-directed mutagenesis, were employed to determine the particular effect of this substitution on fitness. The fitness of E407K strains was evaluated by a multifaceted approach encompassing mycelial growth, sporulation, conidial germination, virulence, acid production, osmotic and oxidative sensitivity, and sclerotial formation and viability. Field isolates carrying the E407K mutation showed decreased sclerotia formation on intermediate medium (IM) and increased conidia production on PDA when compared with sensitive isolates; remarkably, site-directed transformants bearing the same E407K substitution revealed no fitness penalties. The competitive capability of E407K strains on apple fruit was further investigated by employing conidial mixtures at three initial ratios of resistant and susceptible isolates: 19, 11, and 91, respectively. The diminished competitive ability of field-resistant isolates, mirroring fitness limitations, was not observed in site-directed transformants at any initial ratio tested. Field strains resistant to antibiotics (AP) show a fitness disadvantage, according to these results, not a direct consequence of the E407K mutation within the Bcmdl1 gene.A nation's safety depends critically on food security, and the losses caused by pests during food storage create a serious challenge. Regular exposure to chemical pesticides has resulted in a series of serious crises, manifesting as resistance to the pesticides themselves, pesticide residue contamination, environmental pollution, and the danger of exposure for humans and non-target species. A growing interest in the use of volatile components as a natural means to manage storage pests is evident in recent years. Previous research findings show that terpinene-4-ol and limonene demonstrated a substantial insecticidal effect on Sitophilus zeamais, causing considerable effects on CYP450 genes. We investigated the links and functions of related genes and found members of the SzCYP6MS subfamily, which encode a hypothetical protein composed of 493 or 494 amino acids. Exposure to terpinen-4-ol and limonene fumigation led to a substantial increase in the expression of four CYP6MS subfamily genes, as determined by RT-qPCR analysis, when compared to the non-fumigated colonies. Furthermore, our analysis revealed that RNA interference-mediated silencing of CYP6MS genes substantially heightened the susceptibility of *S. zeamais* to terpinen-4-ol and limonene. Specifically, the mortality rates of insects with suppressed CYP6MS1, CYP6MS5, CYP6MS6, CYP6MS8, and CYP6MS9 genes exhibited increases of 25%, 25%, 16%, 17%, and 4% in terpinen-4-ol-treated groups, and 29%, 25%, 15%, 22%, and 3% in limonene-treated groups, respectively, when contrasted with control groups. Ultimately, CYP6MS5's binding to terpinen-4-ol proved the most consistent, mirroring the stability observed in the CYP6MS8-limonene interaction, as confirmed by molecular docking analysis. This study, employing terpinen-4-ol and limonene as novel botanical pesticides, highlights their potential for controlling storage pests, thereby minimizing chemical pesticide use and delaying resistance.Glutathione S-transferases (GSTs) are employed by insects to neutralize a broad spectrum of pesticides, contributing significantly to their detoxification capabilities.