sions or even death.Trichomonas gallinae are parasitic flagellates of importance in wild and domestic birds. The parasite is worldwide distributed, and Columbine birds are its main host. Current research focuses mostly on epidemiological and phylogenetic studies. However, there is still a lack of knowledge regarding parasite-host interaction or therapy development. Real-time PCR is a useful tool for diagnostic and quantification of gene copies in a determined sample. By amplification of a 113-bp region of the 18S small subunit ribosomal RNA gene, a SYBR green-based real-time PCR assay was developed. A standard curve was prepared for quantification analysis. Assay efficiency, linearity, and dissociation analysis were successfully performed. Specificity, sensibility, and reproducibility analysis were tested. This assay could be a useful tool not only for diagnostic purposes but also for future in vivo and in vitro T. gallinae studies. Risk of complications following hernia repair is the key parameter to assess risk/benefit ratio of a technique. As mesh devices are permanent, their risks are life-long. Too many reports in the past assessed mesh safety prematurely after short follow-ups. We aimed to explore what length of follow up would reveal the full extent of complications. Time lapses between implantation and excision were analyzed in 460 cases of meshes excised for complications after hernia repair. Patterns of percentage growth and time lapses at 50th and 95th percentiles were used to compare groups of different hernia type, age, gender and reason for excision. The 50th and 95th case percentiles in the dataset were at 3.75 and 15.0years between mesh implantation and excision. For hernia types, the longest time lapses were for groin hernias (4.0 and 16.11years at 50th and 95th percentiles). The shortest were for umbilical hernias (2.16 and 9.68years). Males had later excisions than females (4.11 and 16.1 vs. 2.47 and 9.79years). o fully assess complications after mesh hernia repair. Especially longer periods are needed to detect mesh erosion into organs and complications in younger males. Presently, short observations and lack of reporting standard in the literature prohibit accurate assessment of complication risks. We propose to use cumulative incidence for standardized risk reporting (y% risk at x years). This will show time-dependent patterns and allow comparisons between different techniques and studies of variable duration. Standardization will also help to predict long-term risks beyond shorter (practical) follow-ups and facilitate real-time monitoring during surveillance.Harvesting waste biomechanical energy has provided a promising approach to improve the power supplement of wearable devices for extending usage life. Surface morphology is a significant factor for enhancing output performance of triboelectric nanogenerator; however, there is a limitation for evaluating the morphology of the surface and its impact on power generation. To evaluate the relationship between the surface morphology and transfer charge, there is a mathematical theory that is the fractal geometry theory that has been proposed to analyze the characteristic of irregular surface morphology. This theory provided a good understanding of the contact area and roughness of the surface. We have designed three categories of knit structures with cord appearance by using a flat knitting machine and analyzed their surface characteristics. Meanwhile, the geometric structures can be demonstrated through the fractal dimension for evaluating the generated output performance during contacting and separation. The present research exhibits that, with the increasing number of knitted units, the triboelectric power-generation performance continued to reduce due to the available contact area decreasing. After calculating the fractal dimension of different knit structures, the m*n rib structures show the high transfer charge when the fractal dimension is close to number one, especially the fractal dimension of the 1*1 rib structure that can reach 0.99. The fractal theory can be further used as an approach to evaluate the influence on the output performance of irregular surface morphology, unrelated to the uniform convex unit distraction. The result of this research also demonstrated the feasibility of a knitted-based triboelectric nanogenerator in scavenging biomechanical energy for powering portable electronics integrated into garments.Rotational spectroscopy plays a major role in the field of observational astrochemistry, enabling the detection of more than 200 species including a plethora of complex organic molecules in different space environments. Those line detections allow correctly determining the sources and physical properties, as well as exploring their morphology, evolutionary stage, and chemical evolution pathways. In this context, quantum chemistry is a powerful tool to the investigation of the molecular inventory of astrophysical environments, guiding laboratory experiments and assisting in both line assignments and extrapolation of the experimental data to unexplored frequency ranges. In the present work, we start by briefly reviewing the rotational model Hamiltonian for asymmetric tops beyond the rigid-rotor approximation, including rotational-vibrational, centrifugal, and anharmonic effects. Then, aiming at further contributing to the recording and analysis of laboratory microwave spectroscopy by means of accessible, less demanding quantum chemical methods, we performed density functional theory (DFT) calculations of the spectroscopic parameters of astrochemically relevant species, followed by their rotational spectrum simulations. Omecamtiv mecarbil Furthermore, dispersion-correction effects combined with different functionals were also investigated. Case studies are the asymmetric tops H2CO, H2CS, c-HCOOH, t-HCOOH, and HNCO. Spectroscopic parameter predictions were overall very close to experiment, with mean percentage errors smaller than 1% for zeroth order and [Formula see text] for first-order constants. We discuss the implications and impacts of those constants on spectrum simulations, and compare line-frequency predictions at millimeter wavelengths. Moreover, theoretical spectroscopic parameters of c-HCOOH and HNCO are introduced for the first time in this work.