Based on the high sensitivity and accuracy mass measurement by TOF/MS, under the optimized extraction condition, the limits of detection (LOD) of this method were obtained in the range of 0.002-0.1 ng ml-1. The linearity was ranged from 0.01 ng ml-1 to 600 ng ml-1, and all the correlation coefficients (R2) were above 0.993. The spiked recoveries were in the range of 80.04% to 109.18% in real sample test and RSD values obtained from 0.95% to 9.85%. The results demonstrate that MF@PDA-PT-μSPE-UHPLC-QTOF is a sample and reliable method for the detection of psychotropic drugs in serum sample.The development of versatile mixed-mode stationary phase materials is of important meanings for solving the increasing demands for real sample analysis. Herein, with 2,5-dihydroxyterephthalic acid as the organic ligand and nickel as the metal centre, MOF-74 nanocrystal materials were facilely grafted on the surface of carboxyl-functionalized silica gel via layer-by-layer assembling technique. The structures of the monodisperse MOF-74@SiO2 material were proved by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, elemental analysis, thermogravimetric analysis, and Brunauer-Emmett-Teller specific surface area and pore size analyzer, respectively. Because the introduced 2,5-dihydroxyterephthalic acid is of hydrophilic carboxyl and hydroxyl groups, the packed MOF-74@SiO2 column reveals hydrophilic interaction/reversed-phase mixed-mode retention properties. Compared with commercial C8 column or silica-based column, the MOF-74@SiO2 column shows distrinct separation selectivity in short separation time for polycyclic aromatic hydrocarbons, phenolic compounds and polar sulfonamide compounds. The developed MOF-74@SiO2 column was further successfully applied for the separation and detection of illegal addition of glucocorticoid in children's face cream as well as sulfonamides veterinary drug residues in pure milk. The research provides a simple and convenient approach to prepare multifunctional MOFs-based stationary phase materials.A systematic methodology was used to quantify ganoderic acid-A (GA-A) loaded nano-lipid carriers (NLC) in rat plasma using UPLC-MS/MS. Separation of the analyte was achieved using ACQUITY UPLC BEH C18 column (1.7 µm) and mobile phase as water containing 0.1% Acetonitrile (40 60% v/v) at a flow rate of 0.4 mL·min-1. The analyte was detected using MRM mode to track precursor-to-product ion transitions of 515.37 → 285.31 m/z (time scan of 2 min) for GA-A, and 175.11 → 115.08 m/z (time scan of 4 min) for ascorbic acid as an internal standard (IS), respectively. The developed method was validated for linearity, accuracy, within and between day precisions, limit of quantification and recovery of the analyte. The results indicated intra and inter-day consistency and precision values were found to be within the acceptance limit for the plasma samples. The method applicability for determination of pharmacokinetic parameters of GA-A was assessed after oral administration of free GA-A solution and GA-A-loaded NLC, which indicated significant difference (p less then 0.05) in the rate and extent of absorption parameters of GA-A from the NLC formulation vis-à-vis the plain solution. Overall, the studies construed successful development and application of UPLC-MS/MS method for estimation of GA-A in the lipidic formulation.Recent technical innovations are revealing surprising patterns in mollusc shell pigmentation, such as an unexpectedly modest role for melanins and rapid divergences in the mix of pigments used to achieve similar colour patterns. The elucidation of the molecular genetic basis of shell pigmentation has been slow, probably because of the high genome complexity of gastropods and bivalves. Recent work within the old field of evolutionary ecology of shell pigmentation allows a greater role for the analysis of large-geographic-scale patterns (sometimes employing citizen-science data), as well as experimental field studies. However, the field remains dominated by land snails as model organisms, while colour pattern evolution in marine gastropods and bivalves, particularly those not exposed to visual predators, remains mysterious. Despite the research and clinical significance of the Postpartum Bonding Questionnaire (PBQ), its psychometric properties have not been studied intensively. Fimepinostat concentration The goodness-of-fit of proposed factor models of the PBQ is poor. Configural and measurement invariance have never been reported. As a secondary analysis of the previous paper (Ohashi et al., 2016), we analysed the PBQ data at 5 days and 1 month after childbirth among 247 mothers of a singleton. We created 9 parcels of PBQ items to perform confirmatory factor analysis (CFA). We also examined configural and measurement invariances of the PBQ factor structure between the two observation times. The CFI of the 3-factor model of the PBQ was .936 and .968 for 5 days and 1 month after childbirth, respectively. Configural, measurement (metric, scalar, and residual), and structural (factor variance and factor covariance) invariances were accepted. The mean of only 'anger and restrictedness' factor was scored higher at 1 month than 5 days after childbirth. The 3-factor model of the PBQ was good in its fit with the data as well as robust in its measurement between the two observation time periods.The 3-factor model of the PBQ was good in its fit with the data as well as robust in its measurement between the two observation time periods.The aim of this study is to develop a novel decellularization method using aqueous extract of soap nut pericarp (SPE) and its evaluation using hematoxylin-eosin staining, scanning electron microscopy, diamidino-2-phenylindol (DAPI) staining, mechanical testing, sodium dodecyl sulfate polyacrylamide gel electrophoresis and DNA quantification. The presently available decellularization agent raises some concerns due to the potential for presence of residual cytotoxic agents in the extracellular matrix. Histological analysis of hematoxylin and eosin and masson's trichrome stained processed aortic samples shows complete decellularization with preservation of extracellular matrix microarchitecture at 120 h. Further, staining of tissue samples with DAPI demonstrates complete removal of DNA fragments. Quantitative evaluation of DNA in the decellularized aorta tissues demonstrated a significant (P 0.05) difference in young's modulus of elasticity, stiffness and stretch ratio between native aortic tissues and decellularized aortic scaffolds.