The interfacial properties depicting the micellization behaviour of the cationic amphiphiles (surfactants) belonging to the class of quaternary ammonium salts varying in degree of hydrophobicity were evaluated using tensiometry, conductivity and fluorescence spectrophotometric methods at 303.15 K. The impact of the amphiphilic nature of these amphiphiles as a function of their concentration is accounted against the selective microbial strains using the well-diffusion approach. Also, its influence on the histological (shrinkage/curling of lamellae, necrosis, haemorrhage, hyperplasia of villi in gills and intestine) alterations and haematological (blood parameters) changes in fingerling of Cirrhinus mrigala (C. mrigala) offers an insight into the stern damages reported as aquatic toxicity. Sodium ascorbate solubility dmso The lesions exhibited moderate to severe alterations that are further correlated with the semi-quantitative mean alteration value (MAV). The in vitro and in vivo findings are explained significantly in terms of amphiphilic hydrophobicity which followed the order C16TAB > C12TAB. All the observed outcomes are rationalized by the structural assessment of the selected amphiphiles as specified by the computational simulation approach using density functional theory (DFT) with B3LYP method and 3-21G basis source set. This work also portrays the biodegradability of these cationic amphiphiles and their fate on the environment. Graphical abstract Molecular architecture of cationic amphiphiles integrated with their in vitro and in vivo rejoinders.Developing a use for the inedible parts of citrus, mainly peel, would have great environmental and economic benefits worldwide. Astaxanthin is a value-added fine chemical that affects fish pigmentation and has recently been used in healthcare products for humans, resulting in an increased demand. This study aimed to produce astaxanthin from a citrus, ponkan, peel extract using the yeast Xanthophyllomyces dendrorhous, which has the ability to use both pentose and hexose. Feeding on only ponkan peel extract enhanced X. dendrorhous growth and the concomitant astaxanthin production. Additionally, we determined that pectin and its arabinose content were the main substrate and sole carbon source, respectively, for X. dendrorhous growth and astaxanthin production. Thus, ponkan peel extract could become a valuable resource for X. dendrorhous-based astaxanthin production. Using citrus peel extract for microbial fermentation will allow the development of processes that produce value-added chemicals from agricultural byproducts.Understanding municipal solid waste (MSW) generation is a key requirement for designing and optimizing MSW collection services. The present contribution proposes a statistical methodology to identify MSW generation patterns from MSW collection records. The methodology aims at finding statistically distinct household waste generation patterns within the days of the week and within months (seasonal variation). It is based on standard statistical methods (ANOVA complemented by non-parametric tests and cluster analysis). The methodology was applied to a Portuguese neighbourhood to assist in the definition of a waste sampling campaign to support the implementation of a pilot PAYT. The results showed the existence of groups with statistically distinct MSW generation patterns both at the weekly and monthly time scales. Three clusters of days of the week, with high, medium and low generation, and two clusters of months, with high and low generation, were identified. These results allowed to design and implement a customized field waste sampling campaign to estimate the MSW generated at the study site with minimal field work. Instead of implementing a homogeneous sampling campaign (equal number of samples for every day of the week and for every month), the samples were collected from the days and months that showed statistically distinct MSW generation pattern. The systematic procedure can be easily adapted to any given location, thus being a useful tool that combines statistical analysis with field collected data.Heavy metal lead is a typical widespread potentially toxic element (PET) contamination due to their extensive and wide applications in industrial processes. The development of cost-effective methods for preventing potentially toxic element lead residues from soil into food is thus highly desirable. A new type of humic acid-based remediation material (HA/WV) incorporating humic acid salt (HA), biochar powder (BC), and wood vinegar (WV), which is a cheap and environmentally friendly industrial by-product from charcoal processing, was prepared and evaluated. The results showed that 0.10 g remediation material HA/WV with a mass ratio of 11 was added to 1 kg surface soil of 0-20 cm from agricultural land contaminated by 300 mg Pb2+, the reduction ratio of available Pb in soil can reach 61.4%. Especially, wood vinegar can enhance the reduction ratio of available Pb by at least 14.7% over without wood vinegar. Furthermore, according to the analysis of adsorption interaction and the electrostatic attraction between Pb(II) and oxygen-containing functional groups on HA/WV are the dominant mechanisms responsible for Pb(II) sorption. The wood vinegar liquid can improve the oxygen-containing group in HA/WV, which can enhance the complexation of remediation materials and Pb(II) ion.The bioavailability of cadmium (Cd) in agricultural soils is a significant health concern due to the potential risk of human exposure via foods grown in Cd-contaminated fields. Biochar has been known to have a highly porous structure and high pH, as well as containing various functional groups; as such, it can immobilize heavy metals. Although it has found that biochar amendment in Cd-contaminated agricultural soils could be effective in reducing Cd bioavailability in previous studies, differences in plant Cd accumulation from Cd-contaminated soils amended with biochars produced from various types of biomass have not been fully discussed yet; we aimed to address this shortcoming in the present work. The soil investigated was an acid soil (pH 5.1) and had an elevated concentration of Cd (total Cd 3.3 mg kg-DW-1). Six kinds of biochar were produced, i.e., from woodchips (Japanese cedar [CE] and Japanese cypress [CY]), moso bamboo (MB), rice husk (RH), poultry manure (PM), and wastewater sludge (WS), at a pyrolysis temperature of 600 °C.