Naringin induces ER stress-mediated apoptosis and simultaneously abrogates Wnt/β-catenin signaling which eventually triggers the arrest of the cell cycle at a G0/G1 phase in CC cells.BACKGROUND Statins produce significant hypolipidemic effects and reduce C-reactive protein (CRP) in patients with chronic obstructive pulmonary disease (COPD). It has been reported that statins did not prevent the acute exacerbation of COPD or improve clinical outcomes. We therefore analyzed the actual therapeutic effects of statins on COPD therapy during long-term clinical trials. METHODS Relevant studies were retrieved from various databases from 2008 to 2019. For each study, odds ratios (ORs), mean difference (MD) and 95% confidence interval (95% CI) were assessed. RESULTS Thirty-two studies were retrieved with 3,137 patients receiving statin therapy and 3,140 controls. Satins significantly increased exercise capacity (47.21, 95% CI 20.79-73.63), lung FEV1 (4.02, 95% CI 2.28-5.75), forced expiratory volume in 1s/forced vital capacity (FEV1/FVC) (3.56, 95% CI 2.01-5.10) and high-density lipoproteins (HDL) (5.573, 95% CI 1.74-9.41). In addition, statins downregulated CRP function (W-1.60, 95% CI -2.45-0.76), IL- 6 (-3.35, 95% CI -4.94--1.76), St George's breath questionnaire (SGRQ) scores (-9.96, 95% CI -12.83--7.10), COPD assessment test (CAT) (-3.49, 95% CI -4.70--2.29) and systolic blood pressure (-4.992, 95% CI -5.17--4.818). Total cholesterol (TC) (-37.84, 95% CI -46.10- 29.58) low-density lipoproteins (LDL) (-26.601, 95% CI -26.688-26.514) and triglycerides (TG) (-42.914, 95% CI -61.809-24.02) were also decreased. CONCLUSIONS Clinical trials conducted over a 10-year period revealed beneficial advantages of statin therapy in COPD patients, permitting increased exercise capacity, FEV1/FVC and HDL. In addition, CRP, IL-6, systolic blood pressure, SGRQ scores and CAT were significantly decreased as well as lipid levels.BACKGROUND Recently, microRNA-99b (miR-99b) shows diverse functions in different human disease. However, further studies about the potential effect of miR-99b in cerebral ischemia injury still need to be done. METHODS The expressions of miR-99b and IGF1R were detected via RT-qPCR assay. Western blot assay was applied to measure the protein expression of Caspase-3, Bax and Bcl-2. MTT assay was used to observe cell viability of SH-SY5Y cells. The association of miR-99b and IGF1R was testified by dual luciferase assay. And human SH-SY5Y cells were treated with the oxygen-glucose deprivation / reperfusion (OGD/R) to mimic CIR injury. RESULTS The expression of miR-99b was increased in the OGD/R model. Prostaglandin Receptor antagonist And upregulation of miR-99b promoted cell viability and inhibited apoptosis induced by OGD/R. Moreover, IGF1R was confirmed as a direct target gene of miR-99b. The expression of IGF1R was obviously decreased under OGD/R conditions. CONCLUSIONS MiR-99b promoted the viability and suppressed apoptosis of SH-SY5Y cells under OGD/R conditions through targeting IGF1R.Polyethylene terephthalate (PET) is a mass-produced synthetic polyester contributing remarkably to the accumulation of solid plastics waste and plastics pollution in the natural environments. Recently, bioremediation of plastics waste using engineered enzymes has emerged as an eco-friendly alternative approach for the future plastic circular economy. Here we genetically engineered a thermophilic anaerobic bacterium, Clostridium thermocellum, to enable the secretory expression of a thermophilic cutinase (LCC), which was originally isolated from a plant compost metagenome and can degrade PET at up to 70°C. This engineered whole-cell biocatalyst allowed a simultaneous high-level expression of LCC and conspicuous degradation of commercial PET films at 60°C. After 14 days incubation of a batch culture, more than 60% of the initial mass of a PET film (approximately 50 mg) was converted into soluble monomer feedstocks, indicating a markedly higher degradation performance than previously reported whole-cell-based PET biodegradation systems using mesophilic bacteria or microalgae. Our findings provide clear evidence that, compared to mesophilic species, thermophilic microbes are a more promising synthetic microbial chassis for developing future biodegradation processes of PET waste. © 2020 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.Lipids are fascinating due to their chemical diversity, which is especially vast in the plant kingdom thanks to the high plasticity of the plant biosynthetic machinery. Lipidomic studies aim to simultaneously analyze a large number of lipid compounds of diverse classes in a given sample. The method presented here uses liquid chromatography-mass spectrometry (LC-MS)-based lipidomic profiling in a relatively fast, robust, and high-throughput manner for high-coverage quantification and annotation of lipophilic compounds. Protocols cover sample preparation, LC-MS-based measurement, and data extraction and annotation. An extensive lipid library for triacylglycerols, galactolipids, and phospholipids is provided. The extended profiling described here could be used in a range of applications and is suitable for integration with other omic datasets. © 2020 by John Wiley & Sons, Inc. Basic Protocol 1 Sample preparation and metabolite extraction Basic Protocol 2 Liquid chromatography-mass spectrometry (LC-MS) analysis Basic Protocol 3 Data extraction, annotation, and quantification.INTRODUCTION Hematology analyzers produce reliable, reproducible, precise, accurate results, as well as a premicroscopic characterization of abnormal samples. We have evaluated the clinical performance of a new blood cell counter, which has been temporarily made available to our hematology laboratory. METHODS Over four months, we analyzed with the Mindray BC-6800 Plus more than 1000 samples with a high incidence of hematological abnormalities, using recommended ICSH and CLSI protocols. We have also assessed flagging efficiency for abnormal cells and scattergram cell distribution. RESULTS From a quantitative point of view, our assessment has identified state-of-the-art level reproducibility, excellent linearity, stability over 48°C at 4°C for the conventional parameters, lack of carry-over ( less then 0.2%), and comparability with the routine instruments. These features would make the instrument suitable for immediate and smooth introduction in the hematology laboratory. Flags for abnormal cells are efficient; flag for blast cells has high sensitivity and predictive value of negative results.