2%) than the unmodified enzyme (60.6%). The current study on B. velezensis SN-14 fibrinolytic enzyme and chemical modification method using Fe2+, mPEG5000, and pepsin provide a reference for developing fibrinolytic drugs and foods.Effect and biosafety are the most noteworthy aspect of the hemostatic materials for trauma treatment. In this work, we evaluated the biocompatibility and hemostatic effect of a novel recombinant collagen hemostatic sponge according to ISO 10993. In addition, the interaction between the recombinant collagen hemostatic sponge and blood cells was observed by scanning electron microscopy, moreover, the hemostatic effect was evaluated by blood clotting assay in vitro and liver hemorrhage models in vivo. As the results, the novel recombinant collagen hemostatic sponge enables to be biodegradable completely in vivo, without stimulation, sensitization, acute toxicity, hematolysis or obvious immune rejection. The procoagulant effect of recombinant hemostatic sponge in vitro is significantly more excellent than that of natural collagen sponge due to the more promotive capacity of blood cell adhesion. Meanwhile, the liver hemorrhage models showed that the hemostatic time of recombinant collagen sponge was 19.33 ± 4.64 s, which was significantly better than that of natural collagen sponge (hemostatic time 31.62 ± 5.63 s). Therefore, the novel recombinant collagen hemostatic sponge with satisfactory biocompatibility and significant hemostatic effect can be performed as a potential novel type of clinical hemostatic products for research and development.Novel degraded potassium channel-modulatory peptides were recently found in thermally processed scorpions, but their pharmacological properties remain unclear. Here, we identified a full-length scorpion toxin (i.e., BmKcug2) and its four truncated analogs (i.e., BmKcug2-P1, BmKcug2-P2, BmKcug2-P3 and BmKcug2-P4) with three conserved disulfide bonds in processed scorpion medicinal material by mass spectrometry. The pharmacological experiments revealed that the recombinant BmKcug2 and BmKcug2-P1 could selectively inhibit the human Kv1.2 and human Kv1.3 potassium channels, while the other three analogs showed a much weaker inhibitory effect on potassium channels. BmKcug2 inhibited hKv1.2 and hKv1.3 channels, with IC50 values of 45.6 ± 5.8 nM and 215.2 ± 39.7 nM, respectively, and BmKcug2-P1 inhibited hKv1.2 and hKv1.3, with IC50 values of 89.9 ± 9.6 nM and 1142.4 ± 64.5 nM, respectively. The chromatographic analysis and pharmacological properties of BmKcug2 and BmKcug2-P1 boiled in water for different times further strongly supported their good thermal stability. Structural and functional dissection indicated that one amino acid, i.e., Tyr36, determined the differential affinities of BmKcug2 and four BmKcug2 analogs. Altogether, this research investigated the different pharmacological properties of BmKcug2 and its truncated analogs, and the findings highlighted the diversity of K+ channel blockers from various scorpion species through thermal processing.The present study demonstrated that chitin-based nanofibers (CNFs) trigger the chitinase genes (PGIP1 and CaChi2), while elevating salicylic acid that can protect plants against pathogens. Cross-talk between this genetic induction and salicylic-acid-mediated immune response was also observed, which may arm a plant against multiple pathovars. Bobcat339 solubility dmso Crab and mushroom based CNFs were synthesized by electrospinning and ball milling techniques. Plants (mung bean, Vigna radiata) (pepper, Capsicum annuum) were pre-inoculated with CNFs and treated with the pathogens Scrolotium rolfsii for pepper and Macrophomina phaseolina for mung bean and shrimp-based CNFs were used as a control. Treated plants had elevated levels of chitinase genes in response to CNFs at inoculation concentrations less then 10 mg/mL both in soil and media, to protect them against the pathogenic fungal disease. After 24 h of exposure to the pathogens, qRT-PCR showed genes class II chitinase gene (CaChi2) and polygalacturonase inhibitor protein 1 (PGIP1) to be up-regulated in both root and shoot at 0.1 and 1 mg/mL of inoculation, respectively. The ball milled mushroom CNFs were sufficient to trigger the membrane based enzymes with less diameter (≥15 nm) to be most efficient versus others. In vitro analysis showed IC50 of ball milled mushroom CNFs to be most efficient in limiting the growth of fungal biomass. Further trigger-like effects were prominent in reducing pathogenic fungal spread in both species.The supply of affordable drinking and sufficiently clean water for human consumption is one of the world's foremost environmental problems and a large number of scientific research works are addressing this issue Various hazardous/toxic environmental contaminants in water bodies, both inorganic and organic (specifically heavy metals and dyes), have become a serious global problem. Nowadays, extensive efforts have been made to search for novel, cost effective and practical biosorbents derived from biomass resources with special attention to value added, biomass-based renewable materials. Lignin and (nano)material adorned lignin derived entities can proficiently and cost effectively remove organic/inorganic contaminants from aqueous media. As low cost of preparation is crucial for their wide applications in water/wastewater treatment (particularly industrial water), future investigations must be devoted to refining and processing the economic viability of low cost, green lignin-derived (nano)materials. Production of functionalized lignin, lignin supported metal/metal oxide nanocomposites or hydrogels is one of the effective approaches in (nano)technology. This review outlines recent research progresses, trends/challenges and future prospects about lignin-derived (nano)materials and their sustainable applications in wastewater treatment/purification, specifically focusing on adsorption and/or catalytic reduction/(photo)degradation of a variety of pollutants.The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA) was fabricated using glycerol as a plasticiser and their optimum blending ratio was identified based on their physical and chemical, structural, mechanical, barrier, and morphological properties. The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis show that both biomacromolecules are well organised due to the hydrogen bond interaction between molecular chains involving the hydroxyl, carbonyl, and acetyl groups. Structural identification was performed by recording X-ray diffraction (XRD) spectra. Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness. The water contact angle of the blend films reached up to 81°, although the value for pure biomacromolecule films was very low. The blend films also exhibited high tensile strength (up to 24 MPa) compared to the pure biopolymer films.