Mix 5 (clove, sage, pomegranate and Cassia fistula) showed the highest inhibition with MIC of 3.0 mg mL-1. This mix exhibited strong anti-bactericidal effect against E. coli O157H7 in meat and fish-fillet products throughout 8 days of cold storage (4°C). The sensory evaluation revealed that Mix 5 was acceptable by panelists with concentration of 0.50% in beef burgers and 0.25% in fish-fillet. CONCLUSION This study suggests that the use of herbal extracts provide antibacterial potentials against food pathogens in meat and fish products.BACKGROUND AND OBJECTIVE Phosphate-solubilising bacteria (PSB) are useful for plant growth. They inhabit different soil ecosystems such as colonizing the root environment. The aims of this study was to isolate PSB from oil palm rhizosphere and to conduct a comparative analysis of the solubility of inorganic phosphates. MATERIALS AND METHODS Rhizospheric soil samples at 0-20 cm depth collected from the distance of 2 m away from the palm were isolated and their chemical and physical properties were analyzed. Pilaralisib Qualitative estimation of the suspected PSB was screened by inoculating and growing them at 27°C for 10 days on NBRIP agar medium with bromophenol blue. Their abilities to solubilize AlPO4, FePO4 and Ca3(PO4)2 were examined. Phosphate solubilizing activities were tested on the NBRIP growth medium by analyzing solubilisation efficiency and soluble-P content. Genomic DNA was isolated using QIAamp® genomic DNA kit. RESULTS A total of 15 PSB were successfully isolated from oil palm rhizosphere. During 5 days of incubation, isolate K3.1, A4 and K3.3 solubilized 53.5, 63.5 and 58.6 mg L-1 phosphate inoculated in Al3PO4, Fe3PO4 and Ca3(PO4)2, respectively. Based on the 16S rRNA gene sequence analysis, those isolates were closely related to Burkholderia arboris, Burkholderia gladioli and Burkholderia seminalis, respectively. In soil analysis, P2O5, C-organic and CEC had positive correlation with the total PSB. CONCLUSION The existence of P promoting bacteria in oil palm rhizosphere may offer effective solution on biofertilizer agent for sustainable agriculture.BACKGROUND AND OBJECTIVE Drying of date helps in preserving it to be consumed outside the harvest season and removes some moisture from dates and also slows down the action of date endogenous enzymes. This study was carried out to investigate pretreatments and drying temperature on the physical and chemical properties of 2 date varieties (Siwi and Sakkoti) at the khalal stage. MATERIALS AND METHODS The date fruits at khalal stage were dipped in ascorbic acid solution, sodium metabisulfite solution and sulfur dioxide before cut into pieces, halves and as whole. Then dates were dried at 50, 55, 60 and 65°C, respectively till ~20% moisture content and examined the physical and chemical properties of dried dates. RESULTS Moisture content of Siwi and Sakkoti at the khalal stage was 56.90 and 51.72%, respectively, while total sugars were 79.76 and 75.74%, respectively on dry weight bases. The color of dates Hunter (L and b) were the highest of treated with meta-bisulfate solution or sulfur dioxide and the lowest of color date observed (Hunter, a) comparing with control and ascorbic acid. CONCLUSION The pretreatments indicated that the dipping dates in sodium meta-bisulfate solution or sulfur dioxide then, dried at 60˚C produce high quality parameters of semi-dry dates comparing control and ascorbic acid.BACKGROUND AND OBJECTIVE Reproduction system is affected by nutrient status of the animal. Flushing is one of reproduction program where the animal should give good quality diet. This study was aimed to evaluate etawah crossbred does reproduction performance giving flushing diet with different fat sources. The fat of plant oils are sunflower and flaxseed and from animal oils are tallow and Lemuru fish. MATERIALS AND METHODS Twenty four of Etawah crossbred does (average body weight 33.83±3.70 kg) were used in this experiment by using completely randomized block design. There are four treatments with four animals of each treatment. The treatments were flushing diet containing 5% sunflower oil (R1), 5.2% flaxseed oil (R2), 5.3% tallow (R3) and 5% Lemuru fish oil (R4). Treatment was given three weeks before and two weeks after matting, following 2 weeks before partus. During pregnant, the does were given basal diet (ratio concentratenapier grass was 7030). Body condition score, nutrient status, blood metabolite and hormone and also performance reproduction were evaluated. RESULTS The nutrient consumption was same in all treatment. Blood glucose were same in all treatments but the highest blood cholesterol was in R3 during estrus and in R4 during mid gestation. The highest plasma estradiol was in R1 during early gestation, while the highest plasma progesterone was in R2 during late gestation. Litter size and birth weight were same in all treatment, while the highest total embryo was in R2 treatment. CONCLUSION It is concluded that flaxseed oil for flushing diet was significantly increased number of total embryo.BACKGROUND AND OBJECTIVE Protein considered the second main nutrient in diet formulation for all types of animals after energy. Present study aimed to evaluate the effect of different treatments on the nutritive value of Jatropha meal (JM) by in vitro and in vivo trials as protein source in sheep diet. MATERIALS AND METHODS Chemical composition, in vitro digestibility, gas production and phorbol esters (PE) were recorded for physically, chemically and biologically treated Jatropha meal. In vivo digestibility was measured by using 24 Barki rams randomly assigned into 4 nutritional groups (6 animals/treatment) as follow 1) control ration and in 2, 3 and 4 groups cotton seed meal replaced with 30, 45 and 60% heated Jatropha meal (HJM). RESULTS The various treatments raised DM (Dry matter), CP (Crude protein), NFE (Nitrogen free extract) and ash, whereas reduced OM (Organic matter), CF (Crude fiber) and EE (Ether extract) content in JM, the results of in vitro dry matter disappearance (IVDMD) have a significant height (p less then 0.01) for physical followed by the chemical and biological treatments. Otherwise high significant results (p less then 0.01) for gas production for different treatments was observed. The different treatments decreased the concentration of PE in JM than untreated. CONCLUSION It can be concluded that all treatments especially heat enhanced chemical composition, IVDMD of JM and gas production. Feeding values were better with the ratio 30 and 45%.