TGF-β3 to seeded cells. HS148 molecular weight The incorporation of GO in PDLLA hydrogel suppressed the burst release of TGF-β3, and significantly prolonged the retention time of the TGF-β3 initially loaded in the hydrogel. Additionally, the GO improved the initial compressive strength of the hydrogel. Both in vitro analyses and in vivo implantation results showed that the GO/PDLLA constructs seeded with human mesenchymal stem cells (hMSCs) showed significantly higher cartilage formation, compared to GO-free scaffolds containing equivalent amount of TGF-β3. Findings from this work suggest the potential application of the GO-TGF/PDLLA hydrogel as a functional scaffold for hMSC-based cartilage tissue engineering. OBJECTIVE To record the definition and management of Very Early Medical Abortion (VEMA) in different countries. STUDY DESIGN An Internet survey was circulated internationally among providers of medical abortion via a website. The questionnaire focused on reasons for performing or delaying medical abortion at a very early gestational age and the perceived advantages and disadvantages of VEMA. RESULTS Out of 220 completed questionnaires, 50 % came from European abortion providers (n = 110). Most respondents (72 %) defined VEMA as abortion performed in the presence of a positive hCG pregnancy test but with an empty uterine cavity or a gestational sac-like structure, and no signs or symptoms of ectopic pregnancy. A total of 74 % of respondents thought it was not necessary to wait for a diagnosis of intrauterine pregnancy before starting medical abortion. Equally, 74 % were aware of the possibility of an ectopic pregnancy. CONCLUSION According to European providers of medical abortion, waiting for the diagnosis of an intrauterine pregnancy is not necessary and does not improve treatment of ectopic pregnancy. Providers should know that medical abortion can be performed effectively and safely as soon as the woman has decided. There is no lower gestational age limit. This study demonstrated the presence of a critical equivalent ratio of the competing anion (i.e., sulfate and bicarbonate) to chloride ion in recycled brine to achieve highly-selective nitrate removal from nitrate-rich groundwater in the standard-anion exchange resin (AER) (i.e., with trimethylamine functional groups) column process. With increasing bicarbonate (or sulfate)chloride equivalent ratio in brine used to circularly activate/regenerate the standard-AER column, considerable bicarbonate (sulfate) removal and dumping were observed. The critical bicarbonate (sulfate)chloride equivalent ratio of 25 (81) in brine effectively achieved zero net bicarbonate (sulfate) removal ( less then 5%) from feedwater during long-term exhaustion-regeneration cyclic operation. The feed rate (6-18 BV/h) played a key role in determining the critical sulfatechloride equivalent ratio in brine, while the feed sulfate concentration (145-345 mg/L) slightly changed the critical sulfatechloride equivalent ratio. The use of optimized ternary brine (with a sulfatechloridebicarbonate equivalent ratio of 4252) stably achieved long-term highly-selective nitrate removal from groundwater in the standard-AER column process with brine electrochemical treatment. The possible mechanism for nitrate selectivity included the modification of the sulfate and bicarbonatechloride equivalent ratios in the standard-AER column by the optimized brine in circular activation/regeneration mode; this changed the column elution and breakthrough curves, inhibited the competition of sulfate and bicarbonate for ion exchange sites during exhaustion according to the separation factor, and finally achieved selective nitrate removal from feedwater. River restoration with the use of in-stream structures has been widely implemented to maintain/improve physical habitats. However, the response of aquatic biota has often been too weak to justify the high costs of restoration projects. The ecological effectiveness of river restoration has thus been much debated over claims that large-scale environmental drivers often overshadow the potential positive ecological effects of locally placed in-stream structures. In this study, we used a two-dimensional hydrodynamic-habitat model to evaluate the ecological effectiveness of habitat restoration with the use of in-stream structures in various water discharges, ranging from near-dry to environmental flows. The habitat suitability of benthic macroinvertebrates and of three cyprinid fish species was simulated for six restoration schemes and at four discharge scenarios, and was compared with a reference model, without in-stream structures. We found that the ecological response to habitat restoration varied by species andn costs-when linked to very specific purposes (i) to conserve endangered species and (ii) to increase/improve habitat availability/suitability during dry periods, thus proactively preventing/reducing the current and future ecological impacts of climate change. Extracellular polymer proteins have been reported to play an important role in enhancing sludge dewaterability during anaerobic digestion in our previous study. However, how the proteins in sludge determine sludge dewaterability remains to be determined. In this work, proteins from digested sludge were identified using label free proteomics analysis, and its hydrophilicity/hydrophobicity properties and functional groups were analysed. We determined that the microbial community variation between the three stages during the anaerobic digestion process was responsible for enhancing sludge dewaterability; The transformation from hydrophilicity to hydrophobicity of digested sludge surface is the result of functional groups distribution variation which caused by the proteins and microbial communities. This study provides a new insight into the development of anaerobic digestion based on sludge dewaterability. Phosphorous (P) recovery from wastewater will become increasingly vital in the future as terrestrial rock phosphate deposits are expended. Effective management of P as a critical resource will require new techniques to recover P from wastewater, ideally in a form that can be used in agriculture as fertiliser. In this study, batch and fixed-bed column conditions were tested using a novel KOH deacetylated calcite-chitosan based adsorbent (CCM) for P removal from aqueous solutions and wastewater effluents. The unique characteristics of this adsorbent as a phosphate adsorbent were the result of rich surface functionality (amine and sulphur functional groups of the chitosan and proteins) and the CaCO3 content (providing donor ligands; and additionally beneficial if the material were used as fertiliser, buffering soil acidification caused by nitrogen application). The maximum P adsorption capacity was determined to be 21.36 mgP/g (at 22 °C) and the endodermic process reached equilibrium after 120 min. The experimental data was best described using a Langmuir isotherm and a pseudo-second order kinetic model.