Moreover, the hyphal and biofilm formation of C. albicans can be blocked by TMOB/FLU@PCM NPs under 808 nm laser irradiation. In vitro and in vivo results indicate that TMOB/FLU@PCM NPs with good biosafety can efficiently eliminate clinical azole-resistant C. albicans. Thus, TMOB/FLU@PCM NPs exhibits a promising future in the treatment of azole-resistant C. albicans infection.The carbon cage of buckminsterfullerene Ih-C60, obeying the Isolated-Pentagon Rule (IPR), can be transformed to the non-IPR C2v-1809C60 cage by a single Stone-Wales rearrangement (SWR) in the course of high-temperature chlorination of C60 with SbCl5. The following high-temperature trifluoromethylation of the chlorination products with CF3I afforded non-IPR CF3 derivatives, 1809C60(CF3)n. X-ray diffraction studies of 1809C60(CF3)n (n = 10, 12, 14, 16) revealed that the sites of pentagon-pentagon fusions on the carbon cage are preferentially occupied by CF3 groups. The addition patterns of 1809C60(CF3)n and related 1809C60Cln are compared, demonstrating a prevailing role of pentagon-pentagon fusions in the stability and structural chemistry of these compounds. Further SWR skeletal transformations of 1809C60 are discussed and compared with the experimental data available.A new one-dimensional model is proposed for the low-energy vibrational quantum dynamics of CH5+ based on the motion of an effective particle confined to a 60-vertex graph Γ60 with a single edge length parameter. Within this model, the quantum states of CH5+ are obtained in analytic form and are related to combinatorial properties of Γ60. The bipartite structure of Γ60 gives a simple explanation for curious symmetries observed in numerically exact variational calculations on CH5+.As inflammation plays a role in the pathogenesis of acute coronary syndromes, C-reactive protein (CRP) can be used as a biomarker. To detect CRP precisely, the authors prepared a CRP electrochemical biosensor consisting of an eight Ag ion-intercalated multifunctional DNA four-way junction (MF-DNA-4WJ) and a porous rhodium nanoparticle (pRhNP) heterolayer on a micro-gap electrode. To increase conductivity, we used eight Ag+ ion-inserted DNA four-way junctions through a C-C mismatch. Each DNA 4WJ was designed to have the CRP aptamer sequence, an anchoring region (thiol group), and two of four C-C mismatch regions at the end of the fragments. After an annealing step, the MF-DNA-4WJ assembly configuration and selective binding of CRP were confirmed through native TBM-PAGE (Tris-borate-magnesium chloride-polyacrylamide gel electrophoresis). The Au micro-gap electrode was fabricated to load 5 μl of the sample, and this was performed during eight experiments on one chip to establish the accuracy of the data. Then, pRhNPs were immobilized on a Au micro-gap electrode using cysteamine. To confirm the electrochemical properties, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were conducted. The durability of pRhNPs was confirmed through CV. To test the sensing performance of the prepared CRP biosensor, the limit of detection (LOD) and selectivity tests were conducted using EIS. The results indicated that charge transfer resistance (Rct) can be used efficiently to probe these interactions within the variable CRP concentration range, from 1 pM to 100 nM (0.23 ng L-1-23 μg L-1). The LOD of this sensor was 0.349 pM (0.08 ng L-1) (at S/N = 3). As a result of diluting the CRP to the same concentration range in a 20% human serum sample, the LOD was 3.55 fM (0.814 pg L-1) (at S/N = 3).A method for the determination of 12 perfluoroalkyl acids (PFAA) in vegetal samples was proposed. The analytical procedure was developed to optimize the detection of short-chain PFAA (C less then 8) due to their higher potential to be translocated and bioaccumulated in plants than long-chain congeners. The method, based on ultrasonic extraction, clean-up and HPLC-MS/MS analysis, determined PFAA in different plant tissues allowing the PFAA distribution and partition in vegetal compartments to be studied. The performance of this analytical procedure was validated by analysing samples (root, stem and leaf) of reed grass. The validated method was then applied to graminaceous plants from an agricultural area impacted by a fluorochemical plant discharge (Northern Italy). The PFAA congeners were detected in most of the samples with ΣPFAA concentrations in the whole plant ranging from less then LOD to 10.4 ng g-1 ww and with a greater rate of PFAA accumulation in corn cob than corn kernel. Ziprasidone research buy The proposed approach is particularly relevant in edible plant investigation because PFAA levels recorded in comestible fractions provide information for human risk assessment due to vegetable consumption. Furthermore data on the remaining not edible parts, intended for forage, are also useful for the assessment of the PFAA transfer in the trophic chain of breeding animals.Industrial discharges resulting in contaminated groundwater is a global environmental problem. For such contaminated groundwater cases, bioremediation is a cost efficient and environmentally friendly approach. The determination and quantification of these pollutants has gained great importance and researchers are currently seeking to develop labor extensive, accurate and reliable methods for evaluating their biodegradation process. In this study, a HPLC method was developed and optimized for the quantification of 11 industrial pollutants studied as two different mixtures benzene, toluene, ethylbenzene, o, m/p-xylene, indane, indene, and naphthalene (mixture A) and benzene, monochlorobenzene, 1,2-dichlorobenzene, and 1,4-dichlorobenzene (mixture B). The method uses two different detectors fluorescence detection and diode array. The fluorescence detector was used for mixture A to achieve lower quantification limits and to quantify separately o-xylene and indene due to them showing similar wavelength behaviors. The limit of detection was found to be between 2 and 70 μg L-1 for mixture A and 290 μg L-1 for mixture B. The limit of quantitation was between 6 and 210 μg L-1 for mixture A and 980 μg L-1 for mixture B, respectively. The novel part of this study is that aqueous samples can be directly measured with one-step sample preparation and it comes with other advantages such as low volumes of sampling from batch bottles and also avoidance of high cost, relative to other analytical techniques. Therefore, this analytical method aims to facilitate the quantification of various aromatic hydrocarbons in laboratory batch samples and can be used as a routine monitoring tool for biological degradation processes of these 11 prevalent contaminants.