Ovarian cancer (OVC), the most lethal form of all gynecological cancers, is a big threat to women's health. Late diagnosis at the advanced stages is one of the major reasons for the ovarian cancer-related deaths. Conventionally, the up-regulated proteins CA125 (cancer antigen 125) and HE4 (human epididymis protein 4) are used as biomarkers to diagnose the OVC malignancies. The lack of sensitivity/specificity and the false-positive results create complexity in the diagnostic process. With specificity over 90 %, HE4 is suitable for diagnosing ovarian cancer. Herein, we have developed an ultrasensitive all-graphene quantum dot (GQD) Förster resonance energy transfer (FRET) probe for the ratiometric detection of HE4 biomarker. A set of two GQD samples were solvothermally prepared and then analyzed by the morphological, structural, and photophysical characterization. One GQD sample exhibited a strong green emission, peaked at around 515 nm, while the other GQD sample displayed a strong red emission with maximum at around 615 nm. The good spectral overlap between the emission and excitation spectra of the green and red GQDs, respectively, all allowed us to consider them for the design of FRET-based probe. 3-deazaneplanocin A research buy The green and red-emitting GQDs were conjugated with HE4 antibody and used as donor and acceptor, respectively for the ratiometric sensing of HE4 ovarian cancer biomarker. The all GQD FRET probe was able to detect as low as 4.8 pM, along with a large dynamic detection range up to 300 nM. The selectivity and interference effect of the developed FRET probe was also investigated against different protein combinations.Albuminuria is a primary feature in patients with CKD and an important contributor to tubulointerstitial fibrosis (TIF) development. Autophagy has been considered to be involved in renal tubular injury caused by albuminuria. Fe3O4 magnetic nanoparticles are related to many cellular activities, such as autophagy and inflammation. Rab7, a molecule involved in both endocytosis and autophagy, has been identified to protect renal tubular epithelial cells from albumin by regulating autophagy and MMP-2 activity in the early stage of albumin stimulation, but its role in the advanced stage is still unclear. Therefore, to investigate the effect of Fe3O4 magnetic nanoparticles on chronic renal tubular injury induced by excess albumin and to further determine the specific role of Rab7, we established a mouse model of TIF by intravenous injection of cationic bovine serum albumin (C-BSA) in Rab7-overexpressing transgenic mice. Our data revealed the decreased autophagy level, weakened MMP-2 activity and exacerbated renal tubular injury in these BSA-overloaded mice; furthermore, the degree of injury was more serious in Rab7-overexpressing transgenic mice. However, the application of Fe3O4 magnetic albumin nanoparticles (Fe3O4@BSA) enhanced MMP-2 activity and alleviated renal tubular injury, and these changes were mediated by an autophagy-dependent mechanism. Taken together, our results indicated that long-term albumin stimulation combined with overexpression of Rab7 could further decrease MMP-2 activity, exacerbate renal tubular injury and accelerate the development of TIF. Fe3O4@BSA could be a promising targeted tool for the management of CKD patients.It is well known that iron oxide magnetic nanoparticles (IONPs) have many potential utilities in biomedicine due to their unique physicochemical properties. With the aim to obtain multifunctional nanoparticles with potential uses for therapy and diagnosis (nanotheranostics), IONPs were synthesized by hydrothermal synthesis assisted by mannose. Two synthetic pathways were evaluated in order to obtain IONPs with suitable properties for biomedical applications. The formulation Mag@Man/H1 presented the best characteristics in terms of size and stability. Mag@Man/H1 was evaluated as a) drug carrier, b) antioxidant activity, c) magnetic hyperthermia, d) contrast agent for MRI. To evaluate the point a), morin, a natural flavonoid with several pharmaceutical activities, was loaded on the nanoparticles. A high percentage of drug loading was achieved. In point b) it was determined that the carrier itself possess a high activity which increased in morin loaded nanoparticles. Point c) magnetocalorimetric evaluation were carried out at several field conditions. A specific absorption rate value of 121.4 W/gFe was achieved at 52.4 kA/m and 260 kHz and 8.8 W/gFe at 4 kA/m and 100 kHz. Regarding contrast capacity (point d), the r1 value found was close to some contrast agent based on manganese. Although the measured r2 value was quite smaller than other iron oxides, the achieved effect was strong enough to produce negative contrast. From these studies, it was concluded that Mag@Man/H1 could act as a multifunctional nanoplatform for oncological diseases treatments.Nanocrystals, due to high drug loading efficiency, have drawn large attention as nanotechnology to enhance solubility and bioavailability of poorly soluble drugs. However, most nanocrystals still encountered low oral absorption percentage due to its insufficient retention time in the gastrointestinal tract (GI). In this work, silybin (SB) as model drug was fabricated to nanocrystals, and further loaded into a mucoadhesive microsphere to increase the GI retention. Such mucoadhesive microspheres were prepared with a wet media milling technique followed by coagulation and film coating. Nanocrystals and microspheres were thoroughly characterized by diverse complementary techniques. As results, such delivery system displayed an encapsulation efficiency of approximately 100 % and a drug loading capacity of up to 35.41 ± 0.31 %. In addition, mucoadhesiveness test ex vivo conducted with rat intestine showed that film-coated microspheres were retained for more than 1 h. Benefiting from nanocrystals technology, the drug cumulative release percentage of the microspheres was remarkable improved compared to unprocessed one in vitro. Finally, pharmacokinetics studies in rats showed a significant 3-fold increase of drug oral bioavailability compared to unprocessed SB. The current study demonstrates that the developed delivery vehicle can enhance the bioavailability of SB by increasing its dissolution percentage as well as through extending retention time in the GI tract, and achieve high drug loading capacity.