Spine curvature measures showed excellent reliability (ICC = 0.79-0.91) and body scaling segments showed fair to excellent reliability (ICC = 0.46-0.95). We found that musculoskeletal models showed mostly excellent between-session reliability to estimate spine loading, with 91% of ICC values > 0.75 for all activities. This information is a necessary precursor for using motion capture data to estimate spine loading from subject-specific musculoskeletal models, and suggests that marker data will deliver reproducible subject-specific models and estimates of spine loading.Conventionally used textile prosthesis for traditional open surgical repair (OSR) of aortic aneurysms have a lower compliance than the native aortic tissue. Graft placements lead to an acute drop in compliance which effects cardiovascular risk and the development of graft related complications. A custom-made spring casing was applied to a Dacron graft segment under physiological pressure conditions within a five-element biventricular mock circulation loop, to investigate experimentally a concept to improve the compliance of a conventional aortic prosthesis by changing the transverse graft cross-section. Two different prosthesis locations, proximal and distal of compliant silicone tubing were used to study uniaxial graft compression with an elastic device. To characterise the devices' performance by means of pulse pressure (PP), diastolic pressure (Pdia) and pulse wave velocity(PWV), fluid pressures and flow were recorded. In a proximal graft setting (ascending aorta repair) elastic uniaxial compression with a custom-made spring casing (2 cm width) could significantly reduce PP by 10-14% (p less then .001) and slowed PWV from 6.7 to 5.2 m/s (22%, p = .002). Applied to a graft in a distal position, the spring casing demonstrated less impact on PP (2-10%), but significantly reduced PWV in this mock aorta segment from 13.7 to 5.5 m/s (60%, p = .004). In conclusion, a newly conceptualised spring casing applied to the external wall of synthetic aortic grafts can reduce PP and slow PWV. By restoring elastic aortic recoil in stiff textile aortic prostheses, the presented concept is a potential solution to improve long-term aortic prosthesis related complications.A simple one-dimensional heterogeneous-source model was used to simulate dissolution of organic liquid that was non-uniformly distributed in physically heterogeneous porous media. The permeability field was depicted as a pseudo-homogeneous medium. The source zone was discretized into multiple domains representing different organic-liquid configurations and hydraulic accessibilities, each with a different representative upscaled mass transfer rate coefficient that is temporally variable. This simplified approach represents a system where minimal information is available regarding system heterogeneities. All factors that influence dissolution were incorporated into the calibrated mass transfer terms. The mass transfer terms were calibrated for each zone separately. The one-dimensional, heterogeneous-source model adequately simulated the multi-stage dissolution behavior observed for column-scale systems that were packed with different natural soils, as well as for flow-cell systems wherein the source zone consisted of both a residual zone and pool. The results indicate that the model adequately simulated the presence of multiple organic-liquid zones in porous media with different configurations and hydraulic accessibilities, which accounts for the non-ideal dissolution behavior observed. The calibrated mass transfer terms for each source type were consistent with those obtained for systems that contained only one of either source type.Dravet syndrome is an epileptic encephalopathy largely due to haploinsufficiency of the voltage-gated sodium channel Nav1.1 that is expressed primarily in GABAergic neurons. Cevidoplenib Syk inhibitor In order to distinguish the different subtypes, we used gene editing to introduce tdTomato gene into the genome of iPSCs to label the GABAergic neurons in the differentiated neuronal networks. The gene-edited cell line demonstrates normal karyotype, expresses the main pluripotency markers, and shows the presence of differentiation into the three embryonic germ layers in teratomas.A human induced pluripotent stem cell (iPSC) line was generated from the urine cells of a 8-year-old female with Gaucher disease with the homozygous changes c.1448 T>C in the GBA gene. Reprogramming factors OCT4, SOX2, KLF4, and miR-302-367 were delivered using a non-integrative plasmid. Our iPSCs showed complete pluripotency, normal genetic stability, and the ability to differentiate into three germ layers.Plane wave expansion (PWE) method is extended to calculate the band structures of a proposed piezoelectric phononic crystal (PC) nanobeam by applying surface elasticity and Timoshenko beam theories. The calculation method is derived and formulized in detail. Further, electrical voltage and external axial force are picked to study the effects of electro-mechanical coupling fields on band gaps, residual surface stress and material intrinsic length are chosen to research the influences of surface effects on band gaps, and ratio between lengths of PZT-4 and epoxy in a unit cell and height-width ratio are picked to investigate the effects of geometric parameters on band gaps. In addition, all the results are compared to those in Euler nanobeam with same parameters. All the results and further analysis demonstrate that the influence rules will play an active role in design process and active control of nano electro-mechanical system based on piezoelectric PC nanobeam.A model describing Listeria innocua evolution according to process parameters of 51 Italian salami processes and HPP in 31 companies was developed. A total of 51 challenge tests were performed. During processing a L. innocua reduction of 0.34-4.32 Log10 CFU/g was observed and HPP further reduced the count of 0.48-3.47 Log10 CFU/g; an overall reduction of 1.04-5.68 is reached. PH after acidification/drying process, aw after seasoning, duration of the seasoning and caliber resulted associated (p less then 0.05) with L. innocua decrease. HPP efficacy was associated (p less then 0.05) with aw and pH of the product higher the pH and aw after the acidification/drying and seasoning phases, higher resulted the L. innocua reduction after HPP. No significant association was observed between L.innocua and salt, nitrate and starter content and other characteristics of process. The model meets companies and Authorities needs and represents a useful tool to predict L. monocytogenes lethality, giving recommendations to food business operators interested in exportation to the U.