PURPOSE To investigate the effect of early treatment of Class II division 1 malocclusion with T4K appliance on soft and hard tissue changes and prognosis of patients with mixed teeth. METHODS Twenty patients with Class II malocclusion, 11 males and 9 females, aged from 9 to 14 years, with an average age of 11.05 years, were selected. All patients were treated with T4K appliance for 12 months. The changes of soft and hard tissues before, 12 months after and 36 months after orthodontic treatment were measured and compared, to explore the stability of T4K appliance in the treatment of Class Ⅱ division 1 malocclusion. The data were analyzed with SPSS 25.0 software package. RESULTS The hard tissue indexes U1-NA, U1-NA, L1-NB, L1-NB were significantly decreased (P0.0.5). CONCLUSIONS T4K appliance used for early treatment of Class Ⅱ malocclusion can correct patients' poor oral habits, improve the relationship between oral and maxillofacial soft and hard tissues, with good long-term efficacy and stable curative effect.PURPOSE To explore and compare the clinical effects of delayed implantation and immediate implantation after minimally invasive extraction. METHODS Eight-six patients from Nanjing Tongren Hospital who underwent minimally invasive extraction of the upper and lower mandibular anterior teeth and premolars from April 2013 to April 2018 were randomly divided into the control group and the experimental group. Forty patients in the control group were treated with delayed implantation after minimally invasive extraction, while 46 in the experimental group underwent immediate implant treatment after minimally invasive extraction. The success rate of the two groups of patients was compared, and the implant stability, the depth of probing around the implant, the aesthetic effect, and the bone level at the edge of the implant and the satisfaction after follow-up were recorded 3 months after implantation and at the day of permanent repair. The data were analyzed using SPSS 20.0 software package. RESULTS After treatment, tthough all the implants have good stability,immediate implantation has higher satisfaction, better aesthetic effect and good clinical application prospects.PURPOSE To measure the density of the cortex in the zygomatic alveolar ridge region of adults by 3D reconstruction, and to analyze the differences of cortical bone density between different vertical facial types in the zygomatic alveolar ridge in different vertical facial types. METHODS Spiral CT scanning data of 48 patients (24 males and 24 females) were chosen for 3D reconstruction and divided into 3 groups(low-angle group, average-angle group and high-angle group) according to the size of the mandibular plane angle (FH-MP), 18 points at which the six planes of the zygomatic alveolar ridge on the left of the upper jaw intersected at 13, 15 and 17 mm above the baseline(the horizontal line of mesial buccal tip of the first maxillary molar) were measured. SPSS 17.0 software package was used for statistical analysis. RESULTS There was no significant difference between genders, there was significant difference among different vertical bone facial types (P less then 0.05）, among which the low-angle group [(1331.65±185.70) HU] was the highest and the high-angle group [(1245.62±207.63) HU] was the lowest. There was significant difference in BMD between different sites in different vertical facial types and the sites with the highest BMD in each group were located at 17 mm above on the occlusion plane. Cross-sectional evaluation showed that the high-angle group was located at the level of the proximal and middle buccal root of the first molar, and the mean-angle group was located between the proximal and distal buccal root, and the low-angle group was located at the level of the distal buccal root of the first molar. CONCLUSIONS There was significant difference in density and distribution of bone cortex in zygomatic alveolar ridge between different vertical facial types in adults, which provides a reference basis for adults to select appropriate site of micro-implant anchorage and to ensure the stability of anchorage in orthodontic treatment.PURPOSE To explore the effect of cooperative training between doctors and nurses on improving the practical ability of micro-endodontics specialist nurses. METHODS Twenty-two nurses in the Department of Endodontics were trained by specialist directors and doctors. The head of department nursing explained in the form of teaching and simulated exercises of medical and nursing training. selleck compound Before and after the training, theoretical and practical abilities of the trained nurses were assessed, and self-made questionnaires were used to investigate the training effect. The results were analyzed by SPSS 10.0 software package. RESUITS Twenty-two trained nurses were compared before and after training in theoretical knowledge and practical ability. Statistical analysis showed that the difference was statistically significant (P less then 0.05). Questionnaire survey showed that 95.5% of trained nurses believed that they could enhance the sense of solidarity and cooperation, 100% of trained nurses believed that they could increase the efficiency of operative therapy, 100% of doctors believed that medical and nursing cooperation training could increase the nursing skill proficiency of trained nurses, and the cooperation of trained nurses was predictable. CONCLUSIONS Cooperative training broadens the specialty knowledge of endodontic specialist nurses, improves their proficiency in cooperation with endodontic specialists and their tacit understanding with doctors, and evidently improves their theoretical and practical abilities.PURPOSE To investigate the correlation between high-risk tumor protein p53 (TP53) mutation and extracapsular spread(ECS) in oral squamous cell carcinoma (OSCC). METHODS The data of 88 OSCC patients admitted to Gansu Provincial People's Hospital from January 2013 to January 2016 were retrospectively analyzed. The patients were divided into non-lymph node metastasis group (A1), lymph node metastasis without ECS group (A2), lymph node metastasis with ECS group(A3) according to the presence or absence of lymph node metastasis. The deletion of exon 5-8 of TP53 gene in primary and metastatic lesions was detected. The correlation of ECS with disease-free survival(DFS), overall survival(OS) rate and TP53 mutation were determined, and single factor analysis of ECS was performed. The data were analyzed by SPSS 20.0 software package. RESULTS TP53 (5) homozygote deletion was found in all three groups. TP53 (6) homozygous deletion was found in group A3 (10/30). No homozygous deletion of TP53(7) was found in three groups.Homozygous deletion of TP53 (8) was found in group A2(7/42), group A3(10/30) and group A3(10/60).