hinitis history, and bedroom carpeting was related to increased asthma symptoms. Further studies are needed to confirm our findings.Family rhinitis history, bedroom carpet, and pollen triggers were the most important risk factors for both asthma and allergy-related symptoms. Our new findings included that hot-water radiator was related to reduced asthma symptoms, and the combination of young age, rhinitis history, and bedroom carpeting was related to increased asthma symptoms. Further studies are needed to confirm our findings.MAX phase materials are a new type of nanomaterial with wide applications, but the potential effects of MAX phase materials on plants have not been reported. Herein, we selected Ti3AlC2 nanosheets as a typical MAX phase material to investigate its potential impacts on rice (Oryza sativa L.) at 0-1000 μg·mL-1. The foliar application of Ti3AlC2 at 100 and 1000 μg·mL-1 inhibited the growth of rice seedlings by producing excess reactive oxygen species (ROS). Furthermore, foliar spraying of Ti3AlC2 at 100 μg·mL-1 decreased the stomatal aperture (78.6%) and increased the number of trichomes (100%). These responses demonstrated that the application of Ti3AlC2 could interfere with the immune system of plants by changing the structure and function of leaves, disturbing the activities of antioxidant enzymes. Selleckchem A-485 According to the above results, we concluded that the toxicity of Ti3AlC2 nanosheets on plants was mainly caused by the release of titanium ions. This study provides a valuable reference for understanding the impact of MAX phase materials on plants.In the framework of the GreenEdge Project (whose the general objective is to understand the dynamic of the phytoplankton spring bloom in Arctic Ocean), lipid composition and viability and stress state of bacteria were monitored in sea ice and suspended particulate matter (SPM) samples collected in 2016 along a transect from sea ice to open water in Baffin Bay (Arctic Ocean). Lipid analyses confirmed the dominance of diatoms in the bottommost layer of ice and suggested (i) the presence of a strong proportion of micro-zooplankton in SPM samples collected at the western ice covered St 403 and St 409 and (ii) a high proportion of macro-zooplankton (copepods) in SPM samples collected at the eastern ice covered St 413 and open water St 418. The use of the propidium monoazide (PMA) method allowed to show a high bacterial mortality in sea ice and in SPM material collected in shallower waters at St 409 and St 418. This mortality was attributed to the release of bactericidal free fatty acids by sympagic diatoms under the effect of light stress. A strong cis-trans isomerization of bacterial MUFAs was observed in the deeper SPM samples collected at the St 403 and St 409. It was attributed to the ingestion of bacteria stressed by salinity in brine channels of ice by sympagic bacterivorous microzooplankton (ciliates) incorporating trans fatty acids of their preys before to be released in the water column during melting. The high trans/cis ratios also observed in SPM samples collected in the shallower waters at St 413 and St 418 suggest the presence of positively or neutrally buoyant extracellular polymeric substances (EPS)-rich particles retained in sea ice and discharged (with bacteria stressed by salinity) in seawater after the initial release of algal biomass. Such EPS particles, which are generally considered as ideal vectors for bacterial horizontal distribution in the Arctic, appeared to contain a high proportion of dead and non-growing bacteria.Phosphorus removal by algae-based biotechnology can be achieved through algal assimilation, surface adsorption, or abiotic precipitation. However, there are still unavailable how these phosphorus removal processes were affected by nanoparticles in wastewater. Here, we employed a non-targeted metabolomic approach to reveal the impact of silver nanoparticles (AgNPs) on the phosphorus assimilation by a unicellular green alga Chlorella vulgaris F1068 (C. vulgaris F1068). Results showed that AgNPs mostly inhibited total phosphorus (TP) removal by the algal assimilation, with TP removal efficiency being reduced by 66.2% (with 0.20 mg/L AgNPs) of the control (without AgNPs). Metabolomics analysis also indicated that AgNPs disturbed metabolic responses related to phosphorus assimilation. AgNPs inhibited phospholipid metabolism which included inositol phosphate metabolism and phosphatidylinositol signaling system (downregulation of glycerol-3-phosphate and myo-inositol, as well as upregulation of serine). Metabolites related to phosphorus assimilation products were impacted through downregulation of guanine, glutamine, alanine, and aspartic acid, as well as upregulation of succinic acid, thereby impeding the algal assimilation of phosphorus. Moreover, perturbation of glutathione metabolism induced by oxidative stress stimulated the alteration of membrane state (upregulation of glycine). These findings contribute to a molecular-scale perspective of nanoparticles on algae-based biotechnology in phosphorus removal.Clomazone, a widely used herbicide, is mainly used in soybean fields. We previously found that clomazone alters Proteobacteria and Nitrospirae abundances and also alters urease activity, which result in changes in NH4+ and NO3- contents in soil nitrogen cycling. It remains unknown, however, how the co-occurrence patterns of species and functions of soil ecosystems change in response to clomazone applications in soil. We designed a 3-month greenhouse experiment to investigate soil microorganism dynamics in response to clomazone. Clomazone was applied at three doses (e.g., T1, T10, T100), which significantly increased bacterial abundance at days 15 and 60. Fungal abundance was stimulated at day 30 in T10-treated soils, whereas fungal abundances decreased in T100-treated soils at day 15. Clomazone altered bacterial and fungal community structures. Network analyses showed more complex and highly connected microbial communities in clomazone-treated soils. Moreover, an Acidobacteria-dominated cluster was identified within each network of clomazone-treated soils. Clomazone applied at the recommended rate decreased the functional groups that were associated with denitrification and hydrogen oxidation at days 15 and 60, and enhanced photoheterotrophy from days 30 to 60. High clomazone inputs increased trophic types (e.g., chemoheterotrophy, phototrophy, photoautotrophy and cyanobacteria) and C cycling functional groups (e.g., fermentation and cellulolysis). The half-life of clomazone ranged from 40.1 to 93.5 days in three cases. Our results provide important information for use of this herbicide.