Compared to E166Cb, E166Cb/N170Q generally exhibited more stable signals owing to its higher deficiency in hydrolyzing the antibiotic analyte. The overall biosensor performance of E166Cb and E166Cb/N170Q was comparable to that of their respective fluorescein-modified counterparts, E166Cf and E166Cf/N170Q. But comparatively, the BADAN-conjugated enzymes showed a higher sensitivity, displayed a faster response in detecting moxalactam and a more stable fluorescence signals towards penicillin G. This study illustrates the potential of BADAN-conjugated β-lactamases as biosensing devices for β-lactam antibiotics.The study of long-lived and regenerative animal models has revealed diverse protective responses to stressors such as aging and tissue injury. Spiny mice (Acomys) are a unique mammalian model of skin wound regeneration, but their response to other types of physiological skin damage has not been investigated. In this study, we examine how spiny mouse skin responds to acute UVB damage or chronological aging compared to non-regenerative C57Bl/6 mice (M. musculus). We find that, compared to M. musculus, the skin epidermis in A. cahirinus experiences a similar UVB-induced increase in basal cell proliferation but exhibits increased epidermal turnover. Notably, A. cahirinus uniquely form a suprabasal layer co-expressing Keratin 14 and Keratin 10 after UVB exposure concomitant with reduced epidermal inflammatory signaling and reduced markers of DNA damage. In the context of aging, old M. musculus animals exhibit typical hallmarks including epidermal thinning, increased inflammatory signaling and senescence. However, these age-related changes are absent in old A. cahirinus skin. Overall, we find that A. cahirinus have evolved novel responses to skin damage that reveals new aspects of its regenerative phenotype.At the beginning of the COVID-19 pandemic (10-14 March, 2020) we conducted a survey (n = 1028) of a nationally representative sample (age, sex, and locale) in Poland. Respondents indicated how strong they thought the threat was to themselves, to Poland, and the world. They also described their emotional reactions to the pandemic, which we used to calculate three scores Anxiety, Hopelessness, and Panic. Respondents also indicated how often they engaged in various coping behaviors and how much they supported different types of economic sacrifice. We used these responses to calculate measures that we labelled as Spread Prevention (e.g., social distancing), Self-preservation (food stockpiling), and Economic Sacrifice (e.g., fighting COVID-19 regardless of the cost). Multiple regression analyses found that perceived threat to self was the most reliable predictor (positive) of emotional reactions and of coping behaviors, and that Anxiety was the most reliable predictor (positive) of Spread prevention and Economic sacrifice. Panic predicted (positively) Self-preservation. A series of mediation analyses found that Anxiety mediated relationships between threat and coping behaviors, and that Panic mediated the relationship between perceived threats and Self-preservation. In addition, we found that scores on all measures, except Panic, increased following the announcement of the first COVID-19 related fatality in Poland, which occurred on the third (middle) day of the study. The mediational relationships we found did not vary as a function of whether the data were collected before or after this announcement. The present results suggest that emotional reactions to perceived threats can serve an instrumental function by providing the motivation to engage in coping behaviors. Such a mechanism complements much research on stress that has focused on how coping mediates threat-emotion relationships.Biofilm-based infections are difficult to treat due to their inherent resistance to antibiotic treatment. Discovering new approaches to enhance antibiotic efficacy in biofilms would be highly significant in treating many chronic infections. Exposure to aminoglycosides induces adaptive resistance in Pseudomonas aeruginosa biofilms. Adaptive resistance is primarily the result of active antibiotic export by RND-type efflux pumps, which use the proton motive force as an energy source. We show that the protonophore uncoupler triclosan depletes the membrane potential of biofilm growing P. click here aeruginosa, leading to decreased activity of RND-type efflux pumps. This disruption results in increased intracellular accumulation of tobramycin and enhanced antimicrobial activity in vitro. In addition, we show that triclosan enhances tobramycin effectiveness in vivo using a mouse wound model. Combining triclosan with tobramycin is a new anti-biofilm strategy that targets bacterial energetics, increasing the susceptibility of P. aeruginosa biofilms to aminoglycosides. In this study, we aimed to study the clinical presentations, and viral clearance of SARS-COV-2 positive quarantined individuals. Cross-sectional study. Governmental- designated facility in the eastern province, Saudi Arabia. 128 laboratory-confirmed COVID-19 quarantined individuals who had a history of travel abroad in the last 14 days before the quarantine or were in direct contact with laboratory-confirmed cases. The study was from March 18th-till April 16th. The clinical presentation, prevalence of asymptomatic carriers among SARS-COV-2 positive quarantined subjects, and the difference between virus clearance among symptomatic and asymptomatic individuals. Sixty-nine of the 128 residents (54%) were completely asymptomatic until the end of the study. The remaining 59 residents (46%) had only mild symptoms. The most common symptom was a sudden loss of smell and taste, accounting for 47.5%. The median time to virus clearance was significantly different between the two groups. Symptomatic residentsfied by contact tracing is high in our study. Therefore, testing, tracing, and isolating travelers and contacts of laboratory-confirmed cases, regardless of symptoms, were very effective measures for early disease identification and containment. Loss of taste and smell were the most common presentations in our mild symptomatic residents and should be used as a screening tool for COVID-19. The persistent positive PCR beyond 14 days observed in the mild symptomatic residents despite being symptoms free, warrant further studies to determine its implications on disease spread and control.