The number of items was reduced through experts' consensus. In the 39-item version, three main dimensions were identified; Cronbach's alpha=0.94; all correlation coefficients were >0.34. Thirteen items were deleted due to poor quality and six items were deleted by the team, generating a 20-item version. Its factorial structure was best reflected with a two-dimension solution (i) social and emotional repercussions; (ii) relation to others, fear of judgement. The Chronic Itch Burden Scale patient-reported questionnaire explores broad aspects of HRQoL relevant for patients with various skin diseases. Its good cross-sectional validity makes it useful for trials and practitioners.The Chronic Itch Burden Scale patient-reported questionnaire explores broad aspects of HRQoL relevant for patients with various skin diseases. Its good cross-sectional validity makes it useful for trials and practitioners.The intervertebral disc (IVD) is a complex tissue, and its degeneration remains a problem for patients, without significant improvement in treatment strategies. This mostly age-related disease predominantly affects the nucleus pulposus (NP), the central region of the IVD. The NP tissue, and especially its microenvironment, exhibit changes that may be involved at the outset or affect the progression of IVD pathology. The NP tissue microenvironment is unique and can be defined by a variety of specific factors and components characteristic of its physiology and function. CM272 in vivo NP progenitor cell interactions with their surrounding microenvironment may be a key factor for the regulation of cellular metabolism, phenotype, and stemness. Recently, celltransplantation approaches have been investigated for the treatment of degenerative disc disease, highlighting the need to better understand if and how transplanted cells can give rise to healthy NP tissue. Hence, understanding all the components of the NP microenvironment seems to be critical to better gauge the success and outcomes of approaches for tissue engineering and future clinical applications. Knowledge about the components of the NP microenvironment, how NP progenitor cells interact with them, and how changes in their surroundings can alter their function is summarised. Recent discoveries in NP tissue engineering linked to the microenvironment are also reviewed, meaning how crosstalk within the microenvironment can be adjusted to promote NP regeneration. Associated clinical problems are also considered, connecting bench-to-bedside in the context of IVD degeneration.Understanding the adaptive changes in maize kernel under high temperature stress (HTS) during grain formation, is critical for developing strategies to alleviate the negative effects of HTS on grain yield and quality. This study performed four temperature treatments on waxy maize, namely, normal day/normal night (control), hot day/normal night, normal day/hot night, and hot day/hot night, from 1 to 15 days after pollination (DAP). Compared to the control, the three HTS treatments inhibited kernel development and starch deposition. To understand how the kernel responded to HTS, the transcriptomes, proteomes and metabolomes of the kernels were studied at 10 and 25 DAPs. Our study found that genes and proteins encoding kernel development and starch deposition were upregulated and downregulated at 10 DAP, respectively, and this expression pattern was reversed at 25 DAP. Metabolome profiling under HTS showed that the accumulation pattern of metabolites at 10 vs. those at 25 DAPs were inversely related. Our multi-omics analyses indicated that the auxin-, abscisic acid-, and salicylic acid-mediated signaling pathways more actively responded to HTS at 10 compared to 25 DAP. The results clarified that the HTS during early kernel development has a carry-over effect on later kernel development. Collectively, the multi-omics profiles of developing kernel under HTS treatment provides insight into the processes that underscore maize yield and quality under high temperature. To determine whether polymorphisms of SLC22A1 and SLCO1B3 genes could predict imatinib (IM) response and chronic myeloid leukemia (CML) risk. We genotyped SLC22A1 (c.480G > C, c.1222A > G) and SLCO1B3 (c.334T > G, c.699G > A) polymorphisms in 132 patients with CML and 109 sex- and age-matched healthy subjects. The patients were evaluated for cytogenetic response by standard chromosome banding analysis (CBA). Polymorphism analysis showed significant increased risk of IM resistance for SLC22A1c.1222AG (P = .03; OR = 2.2), SLCO1B3c.334TT/TG genotypes (P = .007; OR = 4.37) and 334T allele (P = .03; OR = 2.86). The double combinations of SLC22A1c.480CC and c.1222AG polymorphisms with SLCO1B3c.334TT/TG were significantly associated with complete cytogenetic response (CCyR) (P <.05; OR> 7). The interaction between all polymorphisms and smoking were associated with CML development and IM resistance (P ≤.04; OR> 3). Our study results suggest the influence of SLC22A1 and SLCO1B3 polymorphisms and the interaction of smoking on CML development and IM response.Our study results suggest the influence of SLC22A1 and SLCO1B3 polymorphisms and the interaction of smoking on CML development and IM response.The flavonoid content, which is an important indicator of the nutritional value of fruits and vegetables, directly determines the marketability of many fruit crops, including apple (Malus domestica). Brassinosteroids (BRs) are steroid hormones that affect flavonoid biosynthesis in plants, but the underlying regulatory mechanism remains unclear. In this study, treatments with brassinolide (the most active BR) and brassinazole (a BR biosynthesis inhibitor) respectively decreased and increased the flavonoid, anthocyanin, and proanthocyanidin (PA) contents in red-fleshed apple seedlings and calli. We subsequently demonstrated that a BZR family transcription factor (TF), MdBEH2.2, participates in BR-regulated flavonoid biosynthesis. Specifically, MdBEH2.2 inhibits the accumulation of flavonoids, anthocyanins, and PAs in apple seedlings; however, a brassinazole treatment can weaken the inhibitory effect. Additionally, we confirmed that a BR-induced MYB TF, MdMYB60, interacts with MdBEH2.2. The resulting MdBEH2.2-MdMYB60 complex further enhances the inhibitory effect of MdBEH2.