PURPOSE To compare the implant replica position accuracy on a duplicated complete edentulous maxillary implant definitive cast made either using conventional procedures or material jetting additive manufacturing (AM) technology using a coordinate measuring machine (CMM). MATERIAL AND METHODS A complete edentulous maxillary cast with 6 implant replicas was prepared. The definitive cast was duplicated using two manufacturing procedures namely conventional (CNV group) and additive manufacturing procedures (AM group). On the CNV, an AM Co-Cr framework and a custom AM custom tray with a polyether impression material were used to obtain an impression of the definitive cast at room temperature (23°C). On the AM group, the definitive cast was digitized using a laboratory scanner. The standard tessellation language (STL) file was exported and used to manufacture the polymeric AM specimens using a multijet printer following manufacturer´s recommendations. A new digital implant replica was located on each corresponding housing of each AM specimen. A total of 10 specimens per group was obtained. A CMM was selected to measure the position of each implant replica on the definitive cast, CNV, and AM specimens. Linear and angular discrepancies were computed for each specimen. Thus, the Mann Whitney U test was used to analyze the data (p = 0.05). RESULTS There was no significant difference in x-, y-, and z- linear and XZ angular discrepancy between both groups. However, the AM group revealed a significantly higher median YZ angular discrepancy than the CNV group (p = 0.007). CONCLUSIONS Material jetting AM technology demonstrated no significant difference in trueness and precision values of the linear and angular implant replica positions when compared to the conventional technique. © 2020 by the American College of Prosthodontists.Canonically, tRNA synthetases charge tRNAs. However, the lysyl-tRNA synthetase paralog EpmA catalyzes the attachment of (R)-β-lysine to the ε-amino group of lysine 34 of the translation elongation factor P (EF-P) in Escherichia coli. This modification is essential for EF-P-mediated translational rescue of ribosomes stalled at consecutive prolines. In this study, we determined the kinetics of EpmA and its variant EpmA_A298G to catalyze the post-translational modification of K34 in EF-P with eight non-canonical substrates. In addition, acetylated EF-P was generated using an amber suppression system. The impact of these synthetically modified EF-P variants on in vitro translation of a polyproline-containing NanoLuc luciferase reporter was analyzed. Our results show that natural (R)-β-lysylation was more effective in rescuing stalled ribosomes than any other synthetic modification tested. Thus, our work not only provides new biochemical insights into the function of EF-P, but also opens a new route to post-translationally modify proteins using EpmA. This article is protected by copyright. All rights reserved.A primary obligation of medical journals is the responsible, professional, and expeditious delivery of knowledge from researchers and practitioners to the wider community.1 The task of journal editors, therefore, rests not merely in selecting what to publish, but in large measure judging how it might best be communicated. The challenge of improving descriptions of kidney function and disease in medical publishing was the impetus for a Kidney Disease Improving Global Outcomes (KDIGO) Consensus Conference held in June 2019. The conference goals included standardizing and refining kidney-related nomenclature used in English-language scientific articles and developing a glossary that could be used by journals.2. This article is protected by copyright. All rights reserved.The need for extended 2nd field high-resolution (2F-HR) HLA genotyping in kidney transplantation is debated. In a cohort of 1000 kidney transplants, we evaluated the impact of different HLA genotyping levels on the assignment of DSA and investigated whether inference of 2F-HR genotypes from low-resolution (LR) genotypes could be used to correctly assign DSA. Based on LR genotypes, 224 pretransplant DSAs were present in 140 patients and absent in 860 patients (DSAneg group). With extended 2F-HR HLA genotyping, we confirmed 173 DSA (77.2%) in 108 (77.1%) patients (2F-HRpos LRpos DSA group) and excluded DSA in 32 patients (22.9%) (2F-HRneg LRpos DSA group). Kaplan-Meier curves showed that 10-year graft survival rates were similar between the DSAneg and 2F-HRneg LRpos DSA groups (82.4% vs. 93.8%; p=0.27) and confirmed that DSA determined using LR typing but not confirmed using 2F-HR typing, were indeed misclassified. I-BET-762 cell line By inferring 2F-HR genotypes using HaploStats, DSA still could not be correctly assigned in 23.3% of cases. We conclude that extended 2F-HR typing of the donor-recipient pairs is relevant for the correct assessment of DSA. Although inference of 2F-HR genotypes may improve the assessment of DSA in some cases, significant misclassification occurs, and warrants caution in using inferred HLA results for clinical and research purposes. This article is protected by copyright. All rights reserved.BACKGROUND/AIMS Electric-Powered Bikes and powered scooters present a new method of transportation and are becoming commonly used worldwide. However, the reports on traumatic dental injuries related to their use are scarce. The aim of this study was to report the frequency and severity of dental and maxillofacial injuries associated with electric-powered bikes and scooters in Israel between the years 2014-2019. METHODS This was a retrospective cohort study based on data from the Israeli National Trauma Registry (INTR). The INTR provides comprehensive data on hospitalized patients from all six Level I trauma centers (TC) and 15 of the 20 Level II TCs in Israel. All injured patients who were hospitalized due to a traffic collision between 2014-2019 were identified. Then data for those hospitalized due to an e-bike or motorized scooter accident were extracted as well as for pedestrians who were injured as a result of a crash with these vehicles. RESULTS A total of 3,686 hospital admissions were related to electric-powered bikes and scooters.