Resistant pollutants like oil, grease, pharmaceuticals, pesticides, and plastics in wastewater are difficult to be degraded by traditional activated sludge methods. These pollutants are prevalent, posing a great threat to aquatic environments and organisms since they are toxic, resistant to natural biodegradation, and create other serious problems. As a high-efficiency biocatalyst, enzymes are proposed for the treatment of these resistant pollutants. This review focused on the roles and applications of enzymes in wastewater treatment. It discusses the influence of enzyme types and their sources, enzymatic processes in resistant pollutants remediation, identification and ecotoxicity assay of enzymatic transformation products, and typically employed enzymatic wastewater treatment systems. Perspectives on the major challenges and feasible future research directions of enzyme-based wastewater treatment are also proposed.In this study, the anaerobic digestion (AD) applications of early & late biochar dosage were compared for municipal leachate treatment, with the objective of studying the flexible use of biochar as a mitigation measure for biomethane recovery. In two experimental phases, biochar was favourable for the immediate promotion of AD performances, as revealed by Gompertz's model of reduced lag phases, higher biomethane generation rates, and increased biomethane yields. Irrespective of late biochar dosage, it could still retrieve 89% of the ultimate biomethane potential. Comparing the residual VFAs (volatile fatty acids) compositions, it was found that the fraction of long-chain VFAs accounted for 81% of total VFAs in reactor set of early biochar dosage, while it was only 38% in the reactor of late one. Parallel evidence suggested that the schedule of biochar dosage not only could affect methanogenic responses but also the VFAs conversion pathways.The formation of either acetoin or D-2,3-butanediol (D-BDO) by Bacillus amyloliquefaciens cultivated on bakery waste hydrolysates has been evaluated in bioreactor cultures by varying the volumetric oxygen transfer coefficient (kLa). LY411575 concentration The highest D-BDO production (55.2 g L-1) was attained in batch fermentations with kLa value of 64 h-1. Batch fermentations performed at 203 h-1 led to the highest productivity (2.16 g L-1h-1) and acetoin production (47.4 g L-1). The utilization of bakery waste hydrolysate in fed-batch cultures conducted at kLa of 110 h-1 led to combined production of acetoin, meso-BDO and D-BDO (103.9 g L-1). Higher kLa value (200 h-1) resulted to 65.9 g L-1 acetoin with 1.57 g L-1h-1 productivity. It has been demonstrated that the kLa value may divert the bacterial metabolism towards high acetoin or D-BDO production during fermentation carried out in crude bakery waste hydrolysates.Cancer patients are more likely to develop thrombosis, and this co-morbidity is related to the worse prognosis of the disease. The increased formation of neutrophil extracellular traps (NETs) has been proposed as one of the mechanisms to explain cancer-associated thrombosis. In vivo, degradation of NETs with recombinant human DNase I (rhDNase I) prevents thrombus formation in mouse models. In this work, we evaluated the effect of two different chronic treatments with rhDNase I in a murine NET-dependent prothrombotic state in breast cancer model. Medium-term treatment (2.5 mg/kg rhDNase I for eight consecutive days) did not interfere with the primary growth of 4T1 tumors. On the other hand, it effectively prevented thrombus formation in the inferior vena cava stenosis model. Remarkably, medium-term treatment with rhDNase I showed minor impact in the tail-bleeding model. Different from the medium-term, the long-term treatment with rhDNase I (2.5 mg/kg for 18 successive days) drastically reduced the overall survival. Remarkably, the concomitant use of Ertapenem, a carbapenem antibiotic, and rhDNase I significantly attenuated the mortality observed in the long-term treatment. Our results suggest the therapeutic potential of rhDNase I to treat cancer-associated thrombosis, although its chronic use should be carefully evaluated and potentially harmful.Soybean peptides are functional food with good health benefits. The health benefits presented are highly dependent on the peptide structure. In this work, soybean peptides were prepared by alkaline protease hydrolysis of soybean proteins. The peptide structure was identified by UPLC-MS/MS. The full peptide composition was revealed. The sequences of 51 peptides were identified and 46 peptides were assigned as immunomodulatory peptides. By evaluating the immumonodulatory activity and mechanism, soybean peptides could facilitate the proliferation of macrophages. The pinocytotic activity and NO level were increased. Induction of iNOS mRNA expression by soybean peptides was responsible for the increased NO production. The release of cytokines IL-6 and TNF-α was elevated and their levels were equal to positive control. The mRNA expression levels of IL-6 and TNF-α were also improved by soybean peptides, but much lower than positive control. The results were helpful for application of soybean peptides in functional foods.Mutual adulterations of lemon and lime juices may be detected using coumarins and psoralens as markers. Poor manufacturing practices or legal but mechanically intense processing of lemons were recently suspected to lead to false accusations of deliberate adulterations with lime juices due to potentially unspecific markers. Therefore, we studied coumarin and psoralen profiles in carefully dissected flavedo, albedo, and endocarp of lime and lemon as well as in juices produced under variable mechanical stresses at laboratory and pilot plant scale. Although the marker herniarin was detectable in juices from lime and harshly extracted lemons at low levels, isopimpinellin, bergapten and the herein proposed, tentatively assigned 5-geranyloxy-8-methoxypsoralen represented unambiguously lime-specific markers. Coumarin and psoralen data also allowed differentiating juices produced at differing degrees of mechanical stress. The latter was also possible using quantitative 1H-NMR spectroscopy, which yielded best results when combined with HPLC data on coumarins and psoralens.