Recent reports raise the concern that exposure to several environmental chemicals may induce persistent changes that go beyond the exposed organisms, being transferred to subsequent generations even in the absence of the original chemical insult. These changes in subsequent non-exposed generations have been related to epigenetic changes. Although highly relevant for hazard and risk assessment, biomarkers of epigenetic modifications that can be associated with adversity, are still not integrated into hazard assessment frameworks. Here, in order to validate new biomarkers of epigenetic modifications in a popular animal model, zebrafish embryos were exposed to different concentrations of Bisphenol A (0.01, 0.1, 1 and 10 mg/L) and Valproic Acid (0.8, 4, 20 and 100 mg/L), two chemicals reported to alter the modulation of the epigenome. Morphological abnormalities and epigenetic changes were assessed at 80 hours-post fertilization, including DNA global methylation and gene expression of both DNA and histone epigenetic modifications. Gene expression changes were detected at concentrations below those inducing morphological abnormalities. These results further support the importance of combining epigenetic biomarkers with apical endpoints to improve guidelines for chemical testing and hazard assessment, and favour the integration of new biomarkers of epigenetic modifications into the standardized OECD test guideline 236 with zebrafish embryos.Illegal addition of low-dose multi-component residue of hormone-like compounds have sprung up in human activities and production as a cunning strategy to escape from sanitation monitoring, sowing a horrible seed for accidents in food security. Hence, combined strategies with accuracy and efficiency are urgently needed to overcome current circumstance and practical hurdles. Herein, an integrated analysis that compromises recombinant gene yeast bioassay (V400E-YES) and high-performance liquid chromatography-mass spectrometry (HPLC-MS) determination was developed promptly with distinguishing advantage as optimized V400E-YES provides a sum of hormone-like compounds in sample, and subsequent HPLC-MS analysis can accurately quantify the concentration of compounds. In terms of V400E-YES, large-scale random mutagenesis was performed to obtain higher sensitivity hormone receptors. Excitingly, single mutation of estrogen receptor (ER) at V400E and amphimutation of androgen receptor (AR) at G581R/D831E conferred the highest detection sensitivity with a more than 10-fold increase. Subsequently, sensitive HPLC-MS methods for simultaneous detection of eleven estrogens and nine androgens, respectively, were well-constructed. Utilizing this integrated and validated methods, we successfully investigated the hormone residue in environmental samples from farms in Jiangsu, China. Collectively, with multiple estrogens and androgens residue are being widely detected in soil and sewage samples, the feasibility of this method is highlighted, as well as the increasing lurking peril of hormone-like substance residues in environment.Composting has been traditionally considered a process in which a succession of mesophilic and thermophilic microbial populations occurs due to temperature changes. In order to deepen in this model, 1380 bacterial and fungal strains (the entire culturable microbiota isolated from a composting process) were investigated for their ability to grow across a wide range of temperatures (20 to 60 °C). First, qualitative tests were performed to establish a thermal profile for each strain. Then, quantitative tests allowed ascertaining the extent of growth for each strain at each of the tested temperatures. The identity of the isolates enabled to position them taxonomically and permitted tracking the strains throughout the process. Results showed that 90% of the isolates were classified as thermotolerant (they grew at all tested temperatures). Only 9% and 1% of the studied strains showed to be strictly mesophilic or thermophilic, respectively. Firmicutes exhibited the greatest thermal plasticity, followed by Actinobacteria and Ascomycota. Most of the Proteobacteria and all Basidiomycota strains were also able to grow at all the assayed temperatures. Thermotolerance was clearly demonstrated among the composting microbiota, suggesting that the idea of the succession of mesophilic and thermophilic populations throughout the process might need a reassessment.Clean groundwater resources are important for the health of human. In Songyuan City, Northeast China, anthropogenic activities have led to changes in groundwater circulation, thereby depleting the aquifer system and causing water quality deterioration. To evaluate the genesis of water quality, we analyzed the hydrochemical and stable isotope compositions of shallow and deep groundwater. According to drinking water quality standards, the concentrations of Ca2+, Mg2+, and NO3- in 23.0, 30.2 and 35.4% of the samples, respectively, exceeded the acceptable ranges. The groundwater chemistry in these samples was related to geochemical processes and agricultural pollution. find more The hydrochemical analysis explained the reaction mechanisms in each aquifer, showing that the main source of ions in both deep and shallow groundwater is the weathering of silicate rock. In addition, the dissolution of carbonate minerals and artificial pollutants is greater in the shallow groundwater. The stable isotope results showed that long-term extraction is the cause of the diffusion of pollutants in shallow aquifers. Moreover, because most of the well-drilling techniques are backward, the aquifer structure is destroyed, and the deep groundwater is mixed with the shallow groundwater during the process of artificial extraction. The study also analyzed the conditions of the water-rock reaction. Combined with the geological background, it was found that the deep CO2 gas reservoir could provide the necessary material source for the reactions. Owing to frequent tectonic activities, deep CO2 could be discharged to the surface through the fault zone, which promotes the water-rock reaction in this area.