5 × 109-2.6 × 1015 copies/L), respectively. Much as sul 1(log) were dominant over intl 1(log) genes, and their fundamental profiles were also higher in the fish guts than water samples; the Spearman's correlation analyses revealed positive relationship (p Planktomycetes (10-528-25%) featured prominently based on LEfSe use as the hot-spotted biomarkers, hence justifying its higher prospects towards innovative environmental microbiological and biotechnological studies.Diffuse pesticide pollution through tobacco fields is a serious threat to both natural integrities and living beings because tobacco is known as a pesticide-intensive crop. Upsurge in the knowledge of detrimental impacts caused by organochlorine pesticides (OCPs) has made them a burning issue particularly in developing countries. Pakistan is a country famous for its agro-based economy and simultaneously is the second most significant pesticide consumer in South Asian countries. The studied area is tobacco hub of the country. Thus, the present work is aimed to investigate the contamination profile that highlights the ecological and health risk posed by OCPs in River Barandu, located in the proximity of tobacco farming region. ΣOCP levels in sediments ranged between 32.918 and 98.810 ng/g and in water between 0.340 and 0.935 μg/L. Hexachlorocyclohexanes (HCHs) and heptachlor were the most prevailing pesticides in both matrices of the river. Isomeric composition of DDTs and HCHs highlighted that the β-HCH and p,p'-DDT were dominant isomers in water, while α-HCH and p,p'-DDT in sediment compartment. OTS964 concentration Enantiomeric compositions of HCH and DDT indicate both recent and historic uses of these compounds in the area. Indirect contamination through nearby tobacco clusters has been depicted through spatial analysis. Ecological risk assessment based upon the risk quotient (RQ) method revealed that α-endosulfan, dieldrin, heptachlor, and ∑HCHs represent a very high level of ecological risks. The OCPs' lifetime carcinogenic and non-carcinogenic health risks associated with dermal exposure to river's water were considered nominal for surrounding populations. However, detailed ecological and health risk studies are recommended considering the bio-accumulating nature of these contaminants in the food chain.Experiencing outdoor space, especially natural space, during childhood and adolescence has beneficial physical and mental health effects, including improved cognitive and motor skills and a lower risk of obesity. Since school-age children typically spend 35-40 hours per week at schools, we quantified their access to open (non-built-up) space and green space at schools in Greater London. We linked land use information from the UK Ordnance Survey with school characteristics from the Department for Education (DfE) for schools in Greater London. We estimated open space by isolating land and water features within school boundaries and, as a subset of open space, green space defined as open space covered by vegetation. We examined the relationship of both school open and green space with distance to Central London, whether the school was fee-paying, and the percentage of pupils eligible for free school meals (as a school-level indicator of socioeconomic status). Almost 400,000 pupils (30% of all pupils in London) attended schools with less than ten square metre per pupil of open space-the minimum recommended area by DfE-and 800,000 pupils attended schools with less than ten square metre per pupil of green space. Of the latter, 70% did not have any public parks in the immediate vicinity of their schools. School green space increased with distance from Central London. There was a weak association between the school-level socioeconomic indicator and the amount of open and green space. Fee-paying schools provided less open space compared to non-fee-paying schools in central parts of London, but the provision became comparable in suburban London. Many London schools do not provide enough open and green space. There is a need to ensure regular contact with green space through safeguarding school grounds from sales, financially supporting disadvantaged schools to increase their outdoor space and providing access to off-site facilities such as sharing outdoor space with other schools. Bevacizumab with anti-angiogenesis properties reduces the vascular endothelial growth factor (VEGF) level and has widely been used to treat various diseases such as lung diseases and chronic obstructive pulmonary disease (COPD). This study, therefore, aimed to consider the effects of bevacizumab on VEGF receptor 2 (VEGFR2) and lung inflammation of the ovalbumin-induced rat model of airway hypersensitivity. Twenty-one male Wistar rats were randomly divided into 3 groups (n = 7 in each group) (1) control, (2) ovalbumin (OVA)-sensitized, and (3) OVA-sensitized with bevacizumab (OVA + Bmab). Groups 2 and 3 were sensitized with ovalbumin (OVA) and aluminum hydroxide on days 1, 8 and challenged with OVA on day 15 by atomization for 10days (inhalation). After OVA sensitization, the OVA + Bmab was treated with bevacizumab for 2weeks. VEGFR2 was semiquantitatively analyzed in the lungs by immunohistochemistry. VEGF was measured in the lung tissue by ELISA method. The mRNA of IL-10 and IL-6 lung tissue were measured by real-time PCR. Ovalbumin exposure promoted the expression of VEGF and resulted in inflammatory factors overexpression (p ≤ 0.05). However, rats in OVA + Bmab group showed significantly a decrease in VEGFR2 and IL-1β, IL-6, TNFα, and an increase in IL-10 (p ≤ 0.05). The results show that bevacizumab efficiently diminishes bronchial inflammation via reducing the expression of VEGFR2, and IL-6 genes and enhancing the expression of IL-10 gene. Hence, bevacizumab could be considered as a potential candidate drug to control pathological conditions relevant to airway hypersensitivity.The results show that bevacizumab efficiently diminishes bronchial inflammation via reducing the expression of VEGFR2, and IL-6 genes and enhancing the expression of IL-10 gene. Hence, bevacizumab could be considered as a potential candidate drug to control pathological conditions relevant to airway hypersensitivity.