Individual concentration cut-offs are needed for each tube type in all tested biomarkers despite being highly correlated. Serum should be avoided for Aβ42, Aβ40, and t-tau. Freeze-thaw cycles>3 should be avoided for p-tau181. 3 should be avoided for p-tau181. -N370S mutation is one of the most frequent risk factors for dementia with Lewy bodies (DLB) and Parkinson's disease (PD). We looked for genetic variations that contribute to the outcome in N370S-carriers, whether PD or DLB. Whole-genome sequencing of 95 Ashkenazi-N370S-carriers affected with either DLB (n=19) or PD (n=76) was performed, and 564 genes related to dementia and PD analyzed. We identified enrichment of linked alleles in locus in DLB patients (false discovery rate =.0412). Haplotype analysis delineated 1.8 Mb interval encompassing 29 genes and 87 unique variants, of them, -R869C received the highest functional prediction score (Combined Annotation Dependent Depletion =34). Its frequency was significantly higher in 26 DLB-N370S-carriers compared to 140 PD-N370S-carriers (odds ratio [OR]=33.4 =.001, and OR=70.2 when only heterozygotes were included). Because was shown to be important for learning and memory in mice, our data further suggest, for the first time, its involvement in DLB, and possibly in human dementia.Because KIF17 was shown to be important for learning and memory in mice, our data further suggest, for the first time, its involvement in DLB, and possibly in human dementia. The current study explores binge watching as a functional entertainment choice, and examines similarities and differences between it, appointment viewing, and serial viewing in terms of prevalence and technologies used over time. Two surveys were conducted in fall 2015 ( =373, 62% female and the mean age=22.01 [ =5.92]) and in fall 2020 ( =732, 69% female, mean age=21.13 [ =4.98]. Surveys explored the frequency and duration of engaging in binge watching, serial viewing, and appointment viewing, as well as the role of habit in binge watching and the technologies employed to view content. Frequency of binge watching and serial viewing increased from 2015 to 2020, and levels remain elevated from estimates just prior to COVID-19 stay-at-home orders, which saw all types of viewing increase. Participants report most frequently engaging in serial viewing, followed binge watching, followed by appointment viewing. Appointment viewing frequency and duration has decreased. A binge-watching habit explains consiscreen to the living room. During the first wave of COVID-19 it was hypothesized that COVID-19 is subject to multi-wave seasonality, similar to Influenza-Like Illnesses since time immemorial. One year into the pandemic, we aimed to test the seasonality hypothesis for COVID-19. We calculated the average annual time-series for Influenza-Like Illnesses based on incidence data from 2016 till 2019 in the Netherlands, and compared these with two COVID-19 time-series during 2020/2021 for the Netherlands. We plotted the time-series on a standardized logarithmic infection scale. Finally, we calculated correlation coefficients and used univariate regression analysis to estimate the strength of the association between the time-series of COVID-19 and Influenza-Like Illnesses. The time-series for COVID-19 and Influenza-Like Illnesses were strongly and highly significantly correlated. The COVID-19 peaks were all during flu season, and lows were all in the opposing period. Finally, COVID-19 meets the multi-wave characteristics of earlier flu-liies the criteria of earlier respiratory pandemics, namely a first wave that is short-lived at the tail-end of flu season, and a second wave that is longer and more severe.This seems to imply that the same factors that are driving the seasonality of Influenza-Like Illnesses are causing COVID-19 seasonality as well, such as solar radiation (UV), temperature, relative humidity, and subsequently seasonal allergens and allergies.We examined a collection of 386 animal, 451 human, and 109 archived bioaerosol samples with a new pan-species coronavirus molecular assay. Thirty-eight (4.02%) of 946 specimens yielded evidence of human or animal coronaviruses. learn more Our findings demonstrate the utility of employing the pan-CoV RT-PCR assay in detecting varied coronavirus among human, animal, and environmental specimens. This RT-PCR assay might be employed as a screening diagnostic for early detection of coronaviruses incursions or prepandemic coronavirus emergence in animal or human populations.Personalised medicine is based on the principle that each body is unique and will respond to therapies differently. In cardiology, characterising patient specific cardiovascular properties would help in personalising care. One promising approach for characterising these properties relies on performing computational analysis of multimodal imaging data. An interactive cardiac imaging environment, which can seamlessly render, manipulate, derive calculations, and otherwise prototype research activities, is therefore sought-after. We developed the Cardiac Electro-Mechanics Research Group Application (CemrgApp) as a platform with custom image processing and computer vision toolkits for applying statistical, machine learning and simulation approaches to study physiology, pathology, diagnosis and treatment of the cardiovascular system. CemrgApp provides an integrated environment, where cardiac data visualisation and workflow prototyping are presented through a common graphical user interface.Cleft palate patients with a short palate are sometimes encountered and it is difficult to achieve effective primary palatoplasty and good speech in these cases. Our purpose was to establish an effective palatoplasty for a cleft palate patient with Randall type III short palate. Buccal musculomucosal flap on the nasal side and skin graft on the oral side were performed, along with double opposing Z-plasty. Speech improved postoperation. This procedure brought the nasopharyngeal area closer to the normal anatomical state. In terms of disadvantages, the procedure is rather complicated and depends on the engraftment rate.