baltica.A mycotoxicological survey was conducted in breakfast (n = 172) and infant (n = 43) cereals commercialized in Brazil. Samples were collected in 2018 for analyses of aflatoxins (AFs) B1 (AFB1), B2, G1 and G2; fumonisins (FBs) B1 (FB1) and B2; zearalenone (ZEN); the trichothecenes (TRCs) deoxynivalenol (DON), T-2 toxin, HT-2 toxin, nivalenol, fusarenon X, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol and diacetoxyscirpenol; and ochratoxin A. FB1 was the most prevalent metabolite in breakfast cereals, being detected in 26.7% of the samples (mean 105 μg/kg); ZEN had the second highest positivity, 14.8% (mean 17 μg/kg), followed by DON with 10% (mean 44 μg/kg). In infant cereals, FB1 also had the highest incidence, 27.8% (mean 55 μg/kg), followed by DON with 10.3% (mean 36 μg/kg) and ZEN with 6.9% (mean 3 μg/kg). Mycotoxins contamination was found in 31.4% (n = 54) of the breakfast cereals and in 18.6% (n = 8) of the infant cereals. In these positive samples, co-occurrence of two or three mycotoxins was detected in 31.5% (n = 17) of the breakfast cereals and in 25% (n = 2) of the infant cereals. The mycotoxins found co-contaminating the breakfast cereals belong to the genera Aspergillus and Fusarium; ZEN, followed by AFB1, were the most prevalent ones. As for the infant cereals, the associated fungal metabolites are produced by the genus Fusarium; the highest incidence was seen for ZEN. Low contamination and positivity of mycotoxins were found herein; nonetheless, in some samples these substances were present at levels which transgress those preconized in the Brazilian legislation. Therefore, mycotoxicological monitoring of this type of product throughout the nation is crucial in order to identify the potential risk to which the Brazilian population is exposed, particularly the children.The metal(loid)-enriched Avicennia marina biomass obtained from phytoremediation was impregnated with two ferric salts (FeCl3 and Fe(NO3)3) prior to pyrolysis at 300-700 °C, aiming to study the influence on pyrolytic product properties and heavy metal(loid) deportment. Results showed that the impregnated ferric salts increased the fixed carbon content of biochars, hydrocarbon fractions in bio-oils, and the evolution of CO and H2 in gases. Cd in biomass could be effectively removed from the biomass by FeCl3 impregnation. During pyrolysis, the ferric salts enhanced the elemental recovery of As, Cr, Ni and Pb in the biochars and decreased their distribution in gases. Notably, the ferric salt pre-treatment inhibited the mobility and bio-availability of most elements in the biochars. This study indicated that ferric salt impregnation catalysed the pyrolysis process of metal(loid) contaminated biomass, enabled the operation temperature at 500-700 °C with minimal environmental risks, providing a safe and value-added way to the phytoremediation-pyrolysis scheme.Sugarcane straw (SS) is a widely available agricultural processing feedstock with the potential to produce 2nd generation bioethanol and bioproducts, in addition to the more conventional use for heat and/or electrical power generation. In this study, we investigated the operational parameters to maximize the production of xylo-oligosaccharides (XOS) using mild deacetylation, followed by hydrothermal pretreatment. From the laboratory to the pilot-scale, the optimized two-stage pretreatment promoted 81.5% and 70.5% hemicellulose solubilization and led to XOS yields up to 9.8% and 9.1% (w/w of initial straw), respectively. Moreover, different fungal xylanases were also tested to hydrolyze XOS into xylobiose (X2) and xylotriose (X3). GH10 from Aspergillus nidulans performed better than GH11 xylanases and the ratio of the desired products (X2 + X3) increased to 72% due to minimal monomeric sugar formation. Furthermore, a cellulose-rich fraction was obtained, which can be used in other high value-added applications, such as for the production of cello-oligomers.To achieve a sustainable production of food and feed production, inexpensive carbon and nutrient sources are needed. In the present study, biologically upgraded biogas is coupled with electrochemically extracted nitrogen from digested biowaste to cultivate mixed methanotrophs as protein source. Results showed that an increase from less than 5 μgCu2+/L to 100 μgCu2+/L increased the biomass production by 41%. Microbial analysis revealed that the dominated Methylomonas spp. followed by Methylophilus spp. created a specialized community for high CH4 assimilation. Selleckchem GSK1059615 Moreover, duplicate semi-continuous fermenters run for 120 days validating the efficiency of alternative carbon and nitrogen feedstocks at long-term operation. As for dry cell weight (DCW) production, more than 2.5 g-DCW/L were produced using biologically upgraded biogas and electrochemically extracted nitrogen. Furthermore, the protein content and amino acid profile (>50% of DCW) demonstrated that the microbial biomass pose the characteristics to be used as animal feed additive.Ethnopharmacological relevance San-Ye-Tang-Zhi-Qing formula (SYTZQ) is an effective prescription for the treatment of pre-diabetes disorders of glycolipid metabolism in Type 2 Diabetes Mellitus (T2DM). It consists of five Chinese herbs including Folium Mori, Lotus Leaf, Chinese hawthorn leaf, Salvia miltiorrhiza Bunge and Paeoniae Radix Rubra. Aim of the study This study was aimed to reveal the pharmacological mechanism of pharmacokinetic target components of San-Ye-Tang-Zhi-Qing formula for the treatment of Type 2 Diabetes Mellitus. Materials and methods A rapid, precise and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to quantify simultaneously nuciferin, vitexin-4″-O-glucoside, vitexin-2″-O-rhamnoside, paeoniflorin and rosmarinic acid in rat plasma after oral administration of SYTZQ. The network pharmacology was used to analyze the effect of the compounds absorbed into the blood of SYTZQ on Type 2 Diabetes Mellitus. The effects of paeoniflorinpogenicity differentiation in vitro experiments. Conclusions The pharmacokinetic parameters indicated that the five components (nuciferin, vitexin-4″-O-glucoside, vitexin-2″-O-rhamnoside, paeoniflorin and rosmarinic acid) were absorbed and eliminated quickly in vivo. These five absorbed components are associated with 22 pathways, including insulin resistance, regulation of lipolysis in adipocytes, PI3k/AKT-, TNF-, cAMP- and cGMP-PKG-signaling pathway. Paeoniflorin, nuciferine and rosmarinic acid have the effect of inhibiting adipocyte differentiation. This study could provide more reference for quality control, and provide a firm basis for evaluating the clinical efficiency of SYTZQ.