Furthermore, Downregulated miR-448 alleviated oxidative stress injury, and attenuated ASMC cell proliferation, migration and enhanced cell apoptosis through upregulation of PRDM16. Taken together, silencing of miR-448 upregulates PRDM16 and inactivates the TGF-β signaling pathway, thereby impeding development of AS by repressing the proliferation, migration and invasion of ASMCs.[This corrects the article DOI 10.3389/fphys.2020.00234.].[This corrects the article DOI 10.3389/fphys.2020.00099.].The following narrative review addresses the relationship between physical activity and exercise with cardiovascular health, focusing primarily on the following risk factors for atherosclerosis hypertension, dyslipidemia, and vascular function. Cardiovascular diseases are intimately associated with mortality and morbidity, and current societal organization contributes to the incidence of cardiovascular events. A worldwide epidemiological transition to cardiovascular deaths was observed in the last century, with important decrements in physical activity and diet quality. An atherogenic environment started to be the new normal, with risk factors such as dyslipidemia, hypertension, and endothelial dysfunction observed in great portions of the population. Exercise is an important tool to improve overall health. For hypertension, a great amount of evidence now puts exercise as an effective therapeutic tool in the treatment of this condition. The effects of exercise in modifying blood lipid-lipoprotein are less clear. Despite the rationale remaining solid, methodological difficulties impair the interpretation of possible effects in these variables. Vascular function, as assessed by flow-mediated dilatation, is a good measure of overall vascular health and is consistently improved by exercise in many populations. However, in individuals with hypertension, the exercise literature still needs a further description of possible effects on vascular function variables. Physical activity and exercise are associated with improved cardiovascular health, especially with reduced blood pressure, and should be encouraged on the individual and population level. Evidence regarding its effects on blood lipids and flow-mediated dilatation still need solid landmark studies to guide clinical practice.The aim of this study was to investigate inter-day and -week as well as intra- and inter-individual variation of selected biomarkers in high-performance youth soccer players to assist practitioners interpreting player's internal load to counteract underperformance and unwanted health risks. Eleven male youth soccer players were tested multiple times during two 3-week periods at midpoint (3-wkmid) and at the end (3-wkend) of the first half of a German under-19 1. Bundesliga season. The levels of creatine kinase (CK), urea, and C-reactive protein (CRP) were measured during 3-wkmid and 3-wkend each Monday, Wednesday, and Friday. In 3-wkmid the CK median was 14% higher (241 vs. 212 U/L) compared to 3-wkend (P = 0.26, ES = 0.16). Overall, the medians of CK, urea (P = 0.59, ES = 0.08), and CRP (P = 0.56, ES = 0.10) during 3-wkmid did not differ to the values of 3-wkend. Daily coefficient of variations (CVs) ranged from 22 to 71% (CK), 17 to 37% (urea), and 9 to 164% (CRP). Individual medians ranged from 101 to 350 U/L (CK), 23 to 50 mg/dL (urea), and 0.6 to 1.1 mg/L (CRP). High intra-individual variability was demonstrated by large intra-individual CVs (medians CK 50%, urea 18%, and CRP 45%). Our data show (i) large inter-day and inter-week variability of all biomarkers, depending on the external load and (ii) considerable inter- and intra-individual parameter variations. Creatine kinase concentrations could sensitively reflect soccer-specific loads during the season.Arrhythmogenic cardiomyopathy (ACM) is a heritable cardiac disease characterized by fibrotic or fibrofatty myocardial replacement, associated with an increased risk of ventricular arrhythmias and sudden cardiac death. 3-Deazaadenosine cost Originally described as a disease of the right ventricle, ACM is currently recognized as a biventricular entity, due to the increasing numbers of reports of predominant left ventricular or biventricular involvement. Research over the last 20 years has significantly advanced our knowledge of the etiology and pathogenesis of ACM. Several etiopathogenetic theories have been proposed; among them, the most attractive one is the dystrophic theory, based on the observation of similar histopathological features between ACM and skeletal muscle dystrophies (SMDs), such as progressive muscular degeneration, inflammation, and tissue replacement by fatty and fibrous tissue. This review will describe the pathophysiological and molecular similarities shared by ACM with SMDs. There is an increasing interest in the use of eccentric muscle exercise to improve physical condition, especially with regards to its health-related benefits. However, it is known that unaccustomed eccentric exercise causes muscle damage and delayed pain, commonly defined as "delayed onset muscle soreness" (DOMS). The efficacy of herbal preparations in subjects suffering from DOMS has been reported in a few previous studies with small or moderate outcome measures related to muscle recovery. The present study aimed to evaluate the effects of a polyherbal mixture containing whole Roscoe and L., powders called ReWin(d), in young male athletes suffering from DOMS induced by a 1 h session of plyometric exercises. Thirty-three young male athletes participated in this randomized, Triple-blind, placebo-controlled trial 17 of them assigned to the ReWin(d) group and 16 of them to the placebo group. Creatine kinase (CK) was measured as a muscle damage marker, pain was assessed using the Visual Analog Scale (VA maintained the maximum power of the lower limbs and attenuated muscle pain. www.ClinicalTrials.gov, identifier NCT03961022.www.ClinicalTrials.gov, identifier NCT03961022.The tongue is a complex organ involved in a variety of functions such as mastication, speech, and taste sensory function. Enzymatic digestion techniques have been developed to allow the dissociation of the epithelium from the connective tissue of the tongue. However, it is not clear if the integrity and three-dimensional architecture of the isolated epithelium is preserved, and, furthermore if this tissue separation technique excludes its contamination from the mesenchymal tissue. Here, we first describe in detail the methodology of tongue epithelium isolation, and thereafter we analyzed the multicellular compartmentalization of the epithelium by three-dimensional fluorescent imaging and quantitative real-time PCR. Molecular characterization at both protein and transcript levels confirmed the exclusive expression of epithelial markers in the isolated epithelial compartment of the tongue. Confocal imaging analysis revealed that the integrity of the epithelium was not affected, even in the basal layer, where areas of active cell proliferations were detected.