Difficult healing of skin wounds is one of the serious complications of diabetes mellitus. Green tea polyphenols (TP) have been found to have good therapeutic effects on wounds healing. However, TP that is soluble in water and easily been oxidized requires a gel material that provides moisture retention, oxidation prevention, and sustained release of TP to achieve better wound healing effect. Therefore, in this work, novel tea polyphenol nanospheres (TPN) were synthesized and encapsulated in a PVA /alginate hydrogel (TPN@H). The prepared TPN@H was characterized and applicated in model diabetic rats for promoting wound healing and regulating immune response. Fourier-transform infrared spectroscopy (FT-IR), UV spectroscopy, scanning electron microscopy (SEM), atomic force microscope (AFM), confocal laser scanning microscopy (CLSM), dynamic light scattering (DLS) and differential scanning calorimetry (DSC) were used for characterization. Animal experiments and molecular mechanism research proved that TPN@H could promote wound healing of diabetic rats by regulating PI3K/AKT signaling pathway. Bacterial infections of burn wounds are a significant problem that usually slows or stops the process of burn wounds healing. The use of topical antibiotics based on a novel drug delivery system could overcome the limitations of burn wound healing. In this work, the development of new wound dressings based on nanocomposite film of polyvinyl alcohol (PVA) and halloysite nanotubes (HNT) for the delivery of minocycline was investigated. These elastomeric nanocomposites were prepared based on HNT surface modification by APTES and then PVA coating by LbL strategy. The resulting nanocomposites were characterized by FT-IR, XRD, zeta potential, Tg analysis, FESEM, and antibacterial studies. The biodegradability and water uptake of the film were evaluated, the results of which revealed the absorption of scarring and non-degradation of the nanocomposite during treatment. Because minocycline decomposes by light, increasing photostability was another goal that was achieved. The release profile of the drug from the nanocomposite was studied, and it was found to be consistent with the Korsmeyer-Peppas model. In-vitro studies showed the antibacterial effect of nanocomposite on exposure to Gram-positive and Gram-negative bacteria. Due to the properties of the resulting nanocomposite film, it can be considered as a promising candidate for wound healing. buy DCC-3116 In-vivo studies, cell culture, neuroprotective and anti-inflammatory effects may be investigated to develop this wound dressing in the future. Fentanyl is a pain reliever stronger and deadlier than heroin. This lethal drug has killed many people in different countries recently. Due to the importance of the diagnosis of this drug, a fentanyl electrochemical sensor is developed based on a glassy carbon electrode (GCE) modified with the carbon nanoonions (CNOs) in this study. Accordingly, the electrochemical studies indicated the sensor is capable of the voltammetric determination of traces of fentanyl at a working potential of 0.85 (vs. Ag/AgCl). To obtain the great efficiency of the sensor some experimental factors such as time, the potential of accumulation and pH value of the electrolyte were optimized. The results illustrated a reduction and two oxidation peaks for fentanyl in phosphate buffer (PB) with pH = 7.0 under a probable mechanism of electrochemical-chemical-electrochemical (ECE). The differential pulse voltammetry (DPV) currents related to the fentanyl detection were linear with an increase of fentanyl concentrations in a linear range between 1 μM to 60 μM with a detection limit (LOD) of 300 nM. Furthermore, the values of the diffusion coefficient (D), transfer coefficient (α) and catalytic constant rate (kcat) were calculated to be 2.76 × 10-6 cm2 s-1, 0.54 and 1.76 × 104 M-1 s-1, respectively. These satisfactory results may be attributed to utilizing the CNOs in the electrode modification process due to some of its admirable characterizations of this nanostructure including high surface area, excellent electrical conductivity and good electrocatalytic activity. Consequently, these finding points the achieving a simple sensing system to measure of the fentanyl as an important drug from the judicial perspective might be a dream coming true soon. Titanium and its alloys are the most widely used implants in clinical practice. However, their bioactivity is unsatisfactory, and the effect of osteogenesis on the bonding interface between the implant and bone needs to be further improved. In this study, a coating consisting of microporous titanium doped with silicon (Si-TiO2) was successfully created by microarc oxidation (MAO), and Si was evenly distributed on the surface of the coating. The surface morphology, roughness, and phase composition of the Si-TiO2 microporous coating were similar to those of the Si-free doped MAO coatings. The Si-TiO2 microporous coating can promote osteoblast adhesion, spreading, proliferation and differentiation. More importantly, the integrin β1-FAK signaling pathway may be involved in the regulatory effect of the coating on osteoblasts. Further studies in vivo indicated that the Si-TiO2 microporous coating could improve early stage osseointegration. In conclusion, the Si-TiO2 microporous coating is a feasible way to improve the osteogenic abilities of Ti implants to potentially promote clinical performance. Multifunctional nanoparticulate systems, especially those used in medicine, are currently of great interest. In this work, the in-vitro anticancer activity of As4S4/Fe3O4 composites dispersed in a water solution of Poloxamer 407 on breast MCF-7 and tongue SCC-25 cancer cells was verified. An increase in apoptotic cells as a consequence of higher caspase activities, a decrease in mitochondrial membrane potential and an accumulation of cells in the G2/M and subG0/G1 phases were detected after treatment with the As4S4/Fe3O4 nanosuspensions. The sterically stabilized nanosuspensions were characterized in relation to their particle size distribution, zeta potential and long-term stability properties. The interaction between the solid and liquid phases of the nanosuspensions was also studied using Fourier transform infrared spectroscopy.