The findings demonstrate the inappropriateness of existing methods for equivalence appraisal and validate the suggested techniques as reliable and primary tools in correlation analysis.Existing evidence revealed grave prognosis for cryptococcal meningitis (CM), particularly its short-term mortality. However, its long-term survival and prognostic factors remained unknown. This study investigated 3-year mortality and analyzed its predictive factors in patients with CM. This retrospective cohort study with 83 cerebrospinal fluid culture-confirmed CM patients was conducted at China Medical University Hospital from 2003 to 2016. The 3-year mortality rate in patients with CM was 54% (45 deaths among 83 patients). Advanced age, human immunodeficiency virus (HIV) seronegative state, low Glasgow Coma Scale score on admission, decreased hemoglobin and hyperglycemia on diagnosis were associated with 3-year mortality. After multivariate adjustment in the Cox proportional hazard model, only severe hyperglycemia (serum glucose ≥200 mg/dL) on diagnosis could predict 3-year mortality.City air quality monitoring (AQM) network are typically sparsely distributed due to high operation costs. It is of the question of how well it can reflect public health risks to air pollution given the diversity and heterogeneity in pollution, and spatial variations in population density. Combing high-resolution air quality model, spatial population distribution and health risk factors, we proposed a population-health based metric for AQM representativeness. This metric was demonstrated in Hong Kong using hourly modelling data of PM10, PM2.5, NO2 and O3 in 2019 with grid cells of 45m * 48m. Individual and total hospital admission risks (%AR) of these pollutants were calculated for each cell, and compared with those calculated at 16 monitoring sites using the similarity frequency (SF) method. AQM Representativeness was evaluated by SF and a population-health based network representation index (PHNI), which is population-weighted SF over the study-domain. LB-100 The representativeness varies substantially among sites as well as between population- and area-based evaluation methods, reflecting heterogeneity in pollution and population. The current AQM network reflects population health risks well for PM10 (PHNI = 0.87) and PM2.5 (PHNI = 0.82), but is less able to represent risks for NO2 (PHNI = 0.59) and O3 (PHNI = 0.78). Strong seasonal variability in PHNI was found for PM, increasing by >11% during autumn and winter compared to summer due to regional transport. NO2 is better represented in urban than rural, reflecting the heterogeneity of urban traffic pollution. Combined health risk (%ARtotal) is well represented by the current AQM network (PHNI = 1), which is more homogenous due to the dominance and anti-correlation of NO2 and O3 related %AR. The proposed PHNI metric is useful to compare the health risk representativeness of AQM for individual and multiple pollutants and can be used to compare the effectiveness of AQM across cities. Cutaneous leishmaniasis (CL) in Ethiopia, caused by Leishmania aethiopica, is often severe and hard to treat compared to CL caused by other species elsewhere. Miltefosine is the only oral anti-leishmanial drug, with a favorable side-effect profile compared to routinely available sodium stibogluconate (SSG), but evidence about its use for L. aethiopica is lacking. In an observational cohort study, treatment outcomes, safety and adherence among CL patients who required systemic treatment and received miltefosine for 28 days in Boru Meda Hospital and University of Gondar Hospital were studied. Patient cure was defined as 100% flattening for non-ulcerated lesions and 100% flattening and 100% re-epithelization for ulcerated lesions. Outcomes were documented for day 28, 90 and 180, both per site, and pooled, adjusting for site as a fixed effect with effect coding. Among 94 included patients (32 in Gondar, 62 in Boru Meda), median lesion duration was 12 months, median size six cm, and mucosal involvement (46.8%) and diffuse (30.9%) lesions were common. Adherence to miltefosine was good, and side-effects were tolerable. Initial outcomes at day 28 were promising, with 68.8% and 94.0% of patients having good improvement or cure in Gondar and Boru Meda respectively. In Boru Meda, outcomes were good with 72.7% and 72.9% cure at day 90 and day 180 respectively. In Gondar, results were less promising, with only 12.5% and 26.7% cure at day 90 and day 180, although confidence intervals were wide. In pooled estimates, 48.7% of patients reached cure at day 180, and 32.3% relapsed. Outcomes were better in Boru Meda Hospital, for smaller lesions and for mucosal lesions. Based on miltefosine's good initial response, tolerable side-effects, tablet-form, we propose to include miltefosine for future clinical trials using extended treatment schedules, combination therapy, or targeting specific subgroups. ClinicalTrials.gov NCT04004754.ClinicalTrials.gov NCT04004754.Early life respiratory insults, such as viral infections or hyperoxia, often increase asthma susceptibility later in life. The mechanisms underlying this increased susceptibility are not fully understood. IL-33 has been shown to be critically involved in allergic airway diseases. IL-33 expression in the neonatal lung can be increased by various respiratory insults associated with asthma development. Therefore, we investigated whether and how early life increases in IL-33 impact allergic airway responses later in life. Using a novel IL-33 transgenic mouse model, in which full-length IL-33 was inducible overexpressed in lung epithelial cells, we transiently upregulated lung IL-33 expression in neonatal mice for one week. After resting for 4-6 weeks, mice were intranasally exposed to a single-dose of recombinant IL-33 or the airborne allergen Alternaria. Alternatively, mice were exposed to Alternaria and ovalbumin multiple times for one month. We found that a transient increase in IL-33 expression during the neonatal period promoted IL-5 and IL-13 production when mice were later exposed to a single-dose of IL-33 or Alternaria in adulthood. However, increased IL-33 expression during the neonatal period did not affect airway inflammation, type 2 cytokine production, lung mucus production, or antigen-specific antibody responses when adult mice were exposed to Alternaria and ovalbumin multiple times. These results suggest that transient increased IL-33 expression early in life may have differential effects on allergic airway responses in later life, preferentially affecting allergen-induced acute type 2 cytokine production.