Alpha -adrenergic (α -AR) and cannabinoid CB (CB -R) receptors exert their functions modulating multiple signaling pathways, including MEK-ERK (extracellular signal-regulated kinases) and FADD (Fas-associated protein with death domain) cascades. These molecules are relevant in finding biased agonists with fewer side effects, but the mechanisms involving their modulations by α -AR- and CB -R in vivo are unclear. selleck products This study investigated the roles of Gαi and Gαz proteins in mediating α -AR- and CB -R-induced alterations of MEK-ERK and FADD phosphorylation (p-) in mouse brain cortex. Gαi or Gαz protein knockdown was induced in mice with selective antisense oligodeoxinucleotides (ODNs; 3nmol/day, 5days) prior to UK-14,304 (UK or brimonidine; 1mg/kg) or WIN55212-2 (WIN; 8mg/kg) acute treatments. Inactivated (p-T ) MEK1, activated (p-S ) MEK1/2, activated (p-T /Y ) ERK1/2, p-S FADD, and the corresponding total forms of these proteins were quantified by immunoblotting. Increased (+ 88%) p-T MEK1 cortical density, with a concomitant reduction (-43%) of activated ERK was observed in UK-treated mice. Both effects were attenuated by Gαi or Gαz antisense ODNs. Contrastingly, WIN induced Gαi - and Gαz-independent upregulations of p-T MEK1 (+ 63%), p-S MEK1/2 (+ 86%), and activated ERK (+ 111%) in brain. Pro-apoptotic FADD was downregulated (- 34 to 39%) following UK and WIN administration, whereas the neuroprotective p-S FADD was increased (+ 74%) in WIN-treated mice only. None of these latter effects required from Gαi or Gαz protein integrity. The results indicate that α -AR (UK), but not CB -R (WIN), agonists use Gαi and Gαz proteins to modulate MEK-ERK, but not FADD, pathway in mouse brain cortex.The results indicate that α2A-AR (UK), but not CB1-R (WIN), agonists use Gαi2 and Gαz proteins to modulate MEK-ERK, but not FADD, pathway in mouse brain cortex.The present study evaluates the concentration of potentially toxic elements (PTEs), including Pb, Cd, As, Fe, Zn, Cu, and Ni, in vegetable oils through a meta-analytic approach. The published studies in relation to the concentrations of PTEs in vegetable oils were retrieved from major international databases such as PubMed, Scopus, Web of Science, and Embase followed by meta-analysis. Moreover, the health risk assessment was evaluated using total target hazard quotient (TTHQ) by a Monte Carlo simulation (MCS) model. According to the results of 51 articles included among 958 retrieved studies, the concentrations of the PTEs were as follows Cd ≈As (0.110 mg/kg) > Pb (0.086 mg/kg) in cottonseed, canola, and olive oil and Fe (12.964 mg/kg) > Zn (1.044 mg/kg) > Ni (0.893 mg/kg) > Cu (0.264 mg/kg) in cottonseed, olive, and soybean for trace elementals, respectively. Based on the continent type, the higher concentration of Cd, As, and Pb was related to PAHO (American region) and AFRO (African region), and the higher concentrations of Fe, Zn, Cu, and Ni were observed in WPRO (Western Pacific Region), EMRO (Eastern Mediterranean Region), and AFRO. On the other hand, non-carcinogenic health risk assessment of the PTEs indicated that there was a different risk pattern in various countries, and the TTHQ level in adult groups was lower than 1. It can be concluded that the consumption of vegetable oils is safe and does not pose risk to the health of consumers. Sleep disorders are common among nurses and may have negative effects on their performance and well-being. This study aimed to investigate the level of sleep quality and its possible associated factors among hospital-based nurses in Jordan. A cross-sectional design targeting Jordanian hospital-based nurses. In addition to demographics and work habits, the outcome measures included Pittsburgh Sleep Quality Index (PSQI), Nordic Musculoskeletal Questionnaire (NMQ), and Depression Anxiety Stress Scale (DASS). Data were analyzed descriptively and by a multiple variable linear regression analysis to identify predictors of sleep quality. In total, 597 nurses with a mean age of 32.1 (± 5.7) years participated in this study and 47% were men. PSQI mean score was 7.8 (± 3.7) and 68% of the participants showed sleep quality deficits. Stress (β = 0.15 [95%CI 0.13 to 0.19, P < 0.001), depression (β = 0.05 [95%CI 0.009 to 0.08], P = 0.015), the number of joints with 12 months musculoskeletal pain (β = 0.25 [95%CI 0.15 to 0.36], P < 0.001) positively predicted decreased sleep quality. Manual handling training (β = - 0.48 [95%CI -0.96 to 0.003], P = 0.048) and years of experience (β = - 0.05 [95%CI -0.09 to 0.002], P = 0.039) negatively predicted decreased sleep quality. Nurses reported poor levels of sleep quality, high levels of mental health symptoms, and musculoskeletal pain complaints. Sleep quality among nurses and its predictors should be carefully considered by administrations of healthcare services.Nurses reported poor levels of sleep quality, high levels of mental health symptoms, and musculoskeletal pain complaints. Sleep quality among nurses and its predictors should be carefully considered by administrations of healthcare services. Some biomarkers have been reported to be related to the prognosis of the coronavirus disease 2019 (COVID-19). There are sparse data regarding the prognostic value of serum calprotectin in COVID-19 patients. This study aimed to investigate the relationship between serum calprotectin level and clinical severity of COVID-19 disease in hospitalized patients. This retrospective cross-sectional cohort study included 80 consecutive hospitalized patients with confirmed diagnosis of COVID-19. The study population was divided into two groups as patients hospitalized in the intensive care unit (ICU) and patients hospitalized but not in the ICU. The serum calprotectin levels, other laboratory, and clinical parameters were compared between groups. The mean age of the patients was 66.5 ± 15.7years. Of the patients, 42 were in the ICU and 38 were not. Serum calprotectin level and acute-phase reactants such as C-reactive protein, procalcitonin, ferritin, fibrinogen, and white blood cell were significantly higher in ICU patients than in non-ICU patients.