Reference electrodes must maintain a well-defined potential for long periods of time to be useful. The silver/silver chloride (Ag/AgCl) reference electrode is arguably the most widely used reference electrode, but it leaks silver and chloride ions into the sample solution through the porous frit over time. Further, the porous frit makes miniaturization to the micro- and nanoscale challenging. Here, we present an alternative, where the traditional Ag/AgCl reference electrode porous frit is replaced by a conductive wire, preventing ion leakage and allowing miniaturization to the microscale. Charge balance is maintained through a closed bipolar electrochemical mechanism, where faradaic processes occur on each end of the sealed wire. Using the above design, we demonstrate the efficacy of the leakless, bipolar reference electrode (BPRE) and miniaturize it to the microscale (μ-leakless BPRE). Importantly, we demonstrate that leakless and μ-leakless BPREs behave the same as commercial reference electrodes during potentiometric measurements and leakless BPREs perform similarly during voltammetric measurements on ultramicroelectrodes. We demonstrate that the drift during voltammetry using a leakless BPRE on a macroelectrode is slightly more appreciable compared to the drift seen with a commercial reference electrode. We detail design principles for the use of leakless BPREs in nonaqueous solvents and in sealing other conductive materials (e.g., gold and carbon). Using mass spectrometry, we show that the maximum leakage of methylene blue is 0.36 fmol/s, at least 2 orders of magnitude smaller than that of commercial reference electrodes. Finally, we demonstrate the efficacy of using leakless BPREs in potentiometric glucose sensing.Organophosphate esters (OPEs) have been a focus in the field of environmental science due to their large volume production, wide range of applications, ubiquitous occurrence, potential bioaccumulation, and worrisome ecological and health risks. Varied physicochemical properties among OPE analogues represent an outstanding scientific challenge in studying the environmental fate of OPEs in recent years. There is an increasing number of studies focusing on the long-range transport, trophic transfer, and ecological risks of OPEs. Therefore, it is necessary to conclude the OPE pollution status on a global scale, especially in the remote areas with vulnerable and fragile ecosystems. The present review links together the source, fate, and environmental behavior of OPEs in remote areas, integrates the occurrence and profile data, summarizes their bioaccumulation, trophic transfer, and ecological risks, and finally points out the predominant pollution burden of OPEs among organic pollutants in remote areas. Given the relatively high contamination level and bioaccumulation/biomagnification behavior of OPEs, in combination with the sensitivity of endemic species in remote areas, more attention should be paid to the potential ecological risks of OPEs.Despite the huge development of pain management in the past decades, pain remains elusive and many patients still remain in the middle of the ford struggling between low drug efficacy and their overuse. A reason for pain elusiveness is its nature of subjective phenomenon, escaping the meshes of the objectivist, mechanist-reductionist net prevailing in medicine. Actually, pain is not only a symptom but an essential aspect of life, consciousness and contact with the world and its noetic and autonoetic components play a key role in the development of the concepts of pleasure-unpleasure and good-evil. The intensity and tolerability of pain and suffering also depends on what the pain means to the patient. The outstanding effects of placebo and nocebo, behavioral and non-pharmacological techniques warrant the need for a shift from the traditional positivist idea of patient as passive carrier of disease to the patient as active player of recovery and move toward a patient's centered approach exploiting individual resources for recovery. Among the mentioned techniques, hypnosis has proved to increase pain threshold up to the level of surgical analgesia, improve acute and chronic pain as well as coping and resilience, helping to decrease both drug overuse and the costs of pharmacological therapy. find more The wealth of available data suggest the need for a holistic approach, aiming to take care of the individual as an inseparable mind-body unit in its interplay with the environment, where patient's inner world, his/her experience and cognition are taken into due account as powerful resources for recovery through a phenomenological-existential approach. To evaluate the effects of European Resuscitation Council (ERC) COVID-19-guidelines on resuscitation quality emphasizing advanced airway management in out-of-hospital-cardiacarrest. In a manikin study paramedics and emergency physicians performed Advanced-Cardiac-Life-Support in three settings ERC guidelines 2015 (Control), COVID-19-guidelines as suggested with minimum staff (COVID-19-minimal-personnel); COVID-19-guidelines with paramedics and an emergency physician (COVID-19-advanced-airway-manager). Main outcome measures were no-flow-time, quality metrics as defined by ERC and time intervals to first chest compression, oxygen supply, intubation and first rhythm analysis. Data were presented as mean±standard deviation. Thirty resuscitation scenarios were completed. No-flow-time was markedly prolonged in COVID-19-minimal-personnel [113±37sec] compared to Control [55±9sec] and COVID-19-advanced-airway-manager [76±38sec](p<0.001 each). In both COVID-19-groups chest compressions started later [COVID-19-sing the number of staff and by adding an experienced airway manager. The use of endotracheal intubation for reducing aerosol release during resuscitation should be discussed critically as its priorization is associated with an increase in no-flow-time.Delayed chest compressions and prolonged no-flow-time markedly reduced the quality of resuscitation. These negative effects were attenuated by increasing the number of staff and by adding an experienced airway manager. The use of endotracheal intubation for reducing aerosol release during resuscitation should be discussed critically as its priorization is associated with an increase in no-flow-time.