Nanohybrid magnetic optosensing probes were designed and fabricated to enrich and detect ultra-trace levels of mafenide and sulfisoxazole simultaneously. The probes combined the high affinity of MIL-101 and the sensitivity of graphene quantum dots (GQDs) and cadmium telluride quantum dots (CdTe QDs) with the selectivity and rapid separation provided by a magnetic molecularly imprinted polymer (MMIP). Since the MIL101-MMIP-GQD and MIL101-MMIP-CdTe QD probes produced high fluorescence emission intensities at 435 and 572 nm, respectively, mafenide and sulfisoxazole could be simultaneously detected. selleckchem Quantitative analysis was based on fluorescence quenching produced by binding between target molecules and imprinted recognition cavities. In the optimal experimental condition, emission intensity was quenched linearly with increasing analyte concentration from 0.10 to 25.0 μg L-1. Limit of detection was 0.10 μg L-1 for mafenide and sulfisoxazole. The developed optosensor was applied to detect ultra-trace amounts of mafenide and sulfisoxazole in bovine milk. Recoveries of mafenide and sulfisoxazole in spiked bovine milk ranged from 80.4 to 97.9% with RSDs less then 5% and the analysis results agreed well with HPLC analysis. The proposed probes provided excellent sensitivity, selectivity, ease and convenience of use.Whether it is for risk assessment or for remediation purpose, contaminant availability in polluted soils is a key parameter to determine. Two methods were recently standardized for the estimation of the environmental available fraction of non-polar organics but, in some cases, their application on real historically contaminated soils does not provide satisfactory results. The present study aimed at proposing an alternative method for the estimation of PAH availability in soils, based on analytical thermal desorption and molecular analyses with the hypothesis that the binding strength between PAH and the solid matrix is linked to the desorption temperature. This hypothesis was validated by comparing the thermodesorption molecular distribution of different contaminated soils and of their respective extractable organic matter. Then, comparing the thermodesorption profiles of each studied PAH to the efficiency of biological and chemical remediation treatments through principal component analysis allowed obtaining the desorption temperature corresponding to PAH fractions available towards both treatments. This method was proven to effectively estimate the PAH fraction available towards biological (microbial incubation) and chemical (KMnO4 oxidation) treatments and present multiple advantages such as being fast, easy to execute and solvent free.A sensitive and highly reproducible cardiac troponin I (cTnI) immunoassay in human serum is a challenging research goal for researchers studying biosensors because cTnI can undergo proteolysis and various modifications in blood. Furthermore, the reproducible detection of cTnI at very low concentrations is also required for diagnosing acute myocardial infarction. Here, we present sensitive and highly reproducible quartz crystal microbalance (QCM) immunosensors for the detection of cTnI in human serum. The unique features of this study are the use of a pair of capture antibodies that bind to different epitopes of cTnI, and the use of a signal amplification technique that enlarged the size of the titanium dioxide nanoparticles using photocatalytic silver staining. Since QCM measures changes in the resonance frequency due to the changes in mass occurring on the sensor surface, it is possible to quantitatively analyze cTnI based on the enormous increase in mass using a sandwich immunoassay and subsequent signal amplification by silver staining. The detection limit of the cTnI immunoassay in human serum without photocatalytic silver staining was 307 pg/ml, but 18 pg/ml in photocatalytic silver staining-mediated signal amplification. Thus, amplifying the signal increased the sensitivity and reproducibility of the cTnI immunoassay in human serum.Integrating long-term cell culture with real-time electrochemical monitoring is a promising strategy for future studies of physiological and pathological processes. However, great challenges still remain in fabricating such a platform with satisfactory electrochemical performance as well as desirable biocompatibility. Herein, we proposed a novel multifunctional platform based on gold nanoparticles/electrochemically reduced graphene oxide/3-aminopropyl-triethoxysilane modified indium tin oxide plate (ITO/APTES/ErGO/AuNPs). The unique biological and electrical properties of AuNPs and ErGO endow the platform with superior electrocatalytic activity and desirable biocompatibility. As a proof of concept, the present platform showed satisfactory electrochemical performance for sensitive and selective detection of hydrogen peroxide (H2O2) with a sensitivity about 0.25 μA μM-1 cm-2 and a detection limit of 0.38 μM in a linear range of 0.5-1461 μM. And the principle of catalytic reduction was clarified through density functional calculations (DFT). Furthermore, cells grew on the platform exhibited excellent proliferation ability and considerable viability after a long-term cultivation. Based on those desirable performances, in-situ and real-time monitoring of endogenously produced H2O2 released from cancer cells cultured on the platform has been successfully realized, which will be of great significance in pathophysiology research.A novel solid-phase microextraction coating based on polypyrrole (Ppy) with manganese dioxide modified 6-aminohexanoic acid functionalized graphene (MnO2-fGr) and 1-allyl-3-vinylimidazolium bis(trifluoromethylsulfonyl)imide ([AVIm]NTf2) as dopants (Ppy/MnO2-fGr/[AVIm]NTf2) was successfully prepared by electrochemical method. The composite coating was characterized by scanning electron microscope (SEM), Fourier infrared spectrum (FT-IR) and thermogravimetry (TG). The composite coating showed coarse structure, which could improve the specific surface area of it, and according to the TG curve, it also had good thermal stability. The composite coating was used for the headspace-solid phase microextraction (HS-SPME) and chromatographic analysis of benzoates (i.e. methyl benzoate, ethyl benzoate, propyl benzoate, butyl benzoate)，by coupling with gas chromatography with hydrogen flame ionization detector (GC-FID). Under optimal conditions, their enrichment factors were 140-460, linear detection ranges were 25-60000 ng L-1, and detection limits were 2.