The short-term tolerability of *D. polymorpha* to environmental factors might not fully capture the potential for more detrimental outcomes, including mortality, if abiotic stresses, as predicted by climate change models, prove persistent.The widespread presence of silver in nanomaterials has caused an escalation of environmental contamination, posing a danger to the health of aquatic animals. Euryhaline fish, adapted to environments experiencing variable salinity, possess robust osmotic regulation mechanisms to effectively address these fluctuations. This study aimed to investigate the osmoregulatory responses of euryhaline fish to silver, employing medaka (Oryzias latipes) embryos in the experimental design. Immersion in either freshwater (FW) or seawater (SW) solutions containing AgNO3 for 7 days, allowed for the subsequent evaluation of mortality, heart rate, morphology, and ionocyte cell populations within the embryos. The toxicity of AgNO3 exhibited a higher level in FW compared to SW, as evidenced by 50% lethal concentrations (LC50) of 0.17 ppm versus 1.01 ppm. gsk3 signal Embryos exposed to AgNO3 (0.005 and 0.01 ppm) maintained their structural integrity; however, this exposure led to ionocyte dysfunction and an increase in heart rates in freshwater media. Seawater specimens exposed to AgNO3 at 0.1 and 5 ppm concentrations exhibited no changes in morphology, ionocytes, or heart rate. However, embryos transferred from freshwater exhibited impaired hypo-osmoregulation and significantly elevated mortality rates. A twelve-hour delay after the SW transfer revealed severely compromised ionocytes, accompanied by a suppression of water intake, contributing to body dehydration and an overload of sodium. Embryos transferred from saltwater (SW) to freshwater (FW) did not experience a higher rate of mortality in the presence of AgNO3. In summary, the presence of silver in FW during the developmental period of euryhaline fish might jeopardize their survival when transitioning to SW environments.In aquatic environments, tris(13-dichloro-2-propyl) phosphate (TDCIPP) is prevalent, yet its impact on fish intestinal health remains unexplored. Ninety days of exposure to environmentally realistic TDCIPP concentrations (0, 30, 300, and 3000 ng/L) in AB strain zebrafish embryos was followed by evaluation of fish growth, physiological parameters, and intestinal microbial communities using 16S rRNA gene high-throughput sequencing. Zebrafish females exhibited a considerable decline in body length and weight, a change that was not mirrored in male zebrafish. The subsequent analyses of TDCIPP's effects revealed prominent histological intestinal damage, marked by a reduction in tight junction protein 2 (thus impacting the epithelial barrier), increased inflammatory responses (higher interleukin 1 levels), and disruption of neurotransmission (elevated serotonin levels), primarily in the female intestinal tract. Male zebrafish intestinal structures remained intact, and the remarkably elevated activity of glutathione peroxidase (GPx) likely conferred protection against inflammation and resultant intestinal damage. TDCIPP treatment, as determined by 16S rRNA sequencing, caused a sex-dependent alteration of the intestinal microbial community. Specifically, a substantial increase in alpha diversity was observed in the gut microbiome of male zebrafish, which might serve as another protective mechanism against TDCIPP-induced intestinal damage. Elevated counts of pathogenic bacteria (Chryseobacterium, Enterococcus, and Legionella), as revealed by correlation analysis, could partly explain the compromised epithelial barrier function and the observed retardation in the growth of female zebrafish. Our study, uniquely, reveals the profound susceptibility of zebrafish intestinal health and gut microbiota to TDCIPP, especially in female zebrafish, which may contribute in part to the female-biased growth suppression.Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), better known as the cause of COVID-19, is the pathogen responsible for coronavirus disease. The global toll of COVID-19 fatalities, as of September 27th, 2022, reached a staggering 6,541,936. A cytokine storm, marked by the uncontrolled and excessive release of pro-inflammatory cytokines like IL-1, IL-6, NF-κB, and TNF-α from the innate immune system, is a key determinant of SARS-CoV-2 severity. Due to an acute complication, a result of hyper-inflammation, and respiratory organ failure, the patient passed away. Guava, soybeans, and green tea boast bioactive substances with recognized anti-inflammatory and antioxidant effects, potentially offering remedies for the cytokine storm complications of COVID-19. To understand the anti-inflammatory mechanism of green tea, soybean, and guava bioactive compounds, our research is focused on determining their bioactivity, bioavailability, and the target proteins involved in the TNF inhibition pathway. The experiment's design incorporates both in silico methods and the accessibility of datasets. Using the KNApSAck and Dr. Duke databases, samples of bioactive compounds, including their 3D structures and SMILE identities, were obtained. In line with Lipinsky's principles for pharmaceuticals, the SWISSADME web server handled the ADME prediction; the QSAR analysis, on the other hand, was accomplished by the WAY2DRUG web server. The target protein and its protein-protein interactions were scrutinized via STRING DB and Cytoscape software. Ultimately, molecular docking was performed with the aid of Autodock 42, and subsequent visualization was facilitated by BioVia Discovery Studio 2019. Green tea, soybeans, and guava's bioactive compounds, according to the study, are potentially important in anti-inflammation, through their action on the TNF inhibitor pathway.The challenge in developing immunotherapy for colorectal cancer (CRC) lies in finding cell surface targets prominently expressed on cancer cells but with a restricted presence in healthy tissues, to reduce toxicities beyond the tumor. In silico analysis scrutinized a comprehensive list of 13488 CRC-expressed genes, sourced from the Human Protein Atlas (HPA) database, to identify promising surface targets. Criteria included: (i) Absence in brain and lung tissue to mitigate potential neurological and pulmonary toxicity; (ii) Limited expression in other normal human tissues; (iii) Potential encoding of cell surface proteins; and (iv) Moderate expression in CRC cases. Multiple datasets of CRC-derived data were used to shortlist and subsequently rank fifteen potential targets, prioritizing a combination of transcript and protein expression levels. The HPA CRCs, combined with DepMap, TCGA, and CPTAC-2 datasets, were subject to scrutiny. Within the CRC targets, cadherin 17 (CDH17) showed a consistent and markedly high expression, making it the top-ranked target. Downstream analysis of colorectal carcinoma transcriptomic and proteomic data exhibited a significant correlation between the expression of CDH17 and carcinoembryonic antigen. Significantly, CDH17 expression levels were markedly lower in CRC cases associated with high microsatellite instability, and inversely related to immune response gene sets and the expression levels of MHC class I and II molecules. CRCs find a promising therapeutic target in CDH17, and this research offers a novel approach for recognizing pivotal cell surface targets suitable for therapeutic strategies in other malignancies.A subunit of the glycoprotein spikes, found on membrane-enveloped viruses, catalyzes the fusion (the joining together) of the virus's membrane and the membrane of the target cell. This 20-residue N-terminal fusion peptide (Fp) region, part of hemagglutinin subunit 2 in the influenza virus, facilitates binding to the target membrane. A noteworthy question arises concerning the possible membrane changes that might influence the fusion process. Numerous computational investigations have demonstrated a rise in the extent to which lipid acyl chains project outward near the Fp point; in other words, one or more chain carbons are situated closer to the aqueous environment than the headgroup phosphorus. The initial joining of the outer leaflets of the two membranes into an intermediate stalk may be accelerated by protrusion. In this study, a clear rise in the probability of protrusion was observed in membranes incorporating Fp, linked to more pronounced Mn2+-associated increases in the transverse relaxation rates (2's) of the 13C NMR signals from chain segments, in contrast to those membranes without Fp. Lipids near the Fp exhibit a 2:1 ratio compared to those situated further away, as observed through data analysis. The count of Fp-neighboring lipids significantly affects the calculated ratio, and the experimentally validated range of 4 to 24 directly correlates to increased protrusion probabilities across multiple simulations. In the analysis of samples, featuring the presence or absence of Fp, the two observed values are remarkably well-fitted by an exponential decay model as the 13C site progresses closer to the chain's conclusion. Free-energy changes during decay are directly linked to the protrusion's extent, measured by the number of protruded -CH2 units, with each -CH2 group contributing 0.25 kBT of free energy. Analysis of NMR data reveals a prominent role for Fp in the significant extension of lipid chains, wherein the segments adjacent to the headgroups are most likely to protrude.Highly specialized, lipid-storing cells, white adipocytes, are a critical component of the body. The large cell size and high buoyancy of adipocytes are among the unique characteristics that have made studying them in vitro challenging. Traditional monolayered adipocyte culture models frequently fail to accurately replicate the morphology and gene expression profiles of mature adipocytes. The recent creation of 3D adipocyte cultures displays a potential to sidestep significant drawbacks, and holds considerable promise for better in vitro studies of adipocytes. Among the key advances are 3D adipose tissue models, vascularized and immunocompetent, and the innovative organ-on-a-chip models.